TY  - JOUR
A1  - Schilcher, Felix
A1  - Hilsmann, Lioba
A1  - Rauscher, Lisa
A1  - Değirmenci, Laura
A1  - Krischke, Markus
A1  - Krischke, Beate
A1  - Ankenbrand, Markus
A1  - Rutschmann, Benjamin
A1  - Mueller, Martin J.
A1  - Steffan-Dewenter, Ingolf
A1  - Scheiner, Ricarda
T1  - In vitro rearing changes social task performance and physiology in honeybees
JF  - Insects
N2  - In vitro rearing of honeybee larvae is an established method that enables exact control and monitoring of developmental factors and allows controlled application of pesticides or pathogens. However, only a few studies have investigated how the rearing method itself affects the behavior of the resulting adult honeybees. We raised honeybees in vitro according to a standardized protocol: marking the emerging honeybees individually and inserting them into established colonies. Subsequently, we investigated the behavioral performance of nurse bees and foragers and quantified the physiological factors underlying the social organization. Adult honeybees raised in vitro differed from naturally reared honeybees in their probability of performing social tasks. Further, in vitro-reared bees foraged for a shorter duration in their life and performed fewer foraging trips. Nursing behavior appeared to be unaffected by rearing condition. Weight was also unaffected by rearing condition. Interestingly, juvenile hormone titers, which normally increase strongly around the time when a honeybee becomes a forager, were significantly lower in three- and four-week-old in vitro bees. The effects of the rearing environment on individual sucrose responsiveness and lipid levels were rather minor. These data suggest that larval rearing conditions can affect the task performance and physiology of adult bees despite equal weight, pointing to an important role of the colony environment for these factors. Our observations of behavior and metabolic pathways offer important novel insight into how the rearing environment affects adult honeybees.
KW  - honeybee
KW  - artificial rearing
KW  - behavior
KW  - in vitro
KW  - juvenile hormone
KW  - triglycerides
KW  - PER
KW  - foraging
KW  - nursing
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-252305
SN  - 2075-4450
VL  - 13
IS  - 1
ER  - 
TY  - JOUR
A1  - Mayr, Antonia V.
A1  - Keller, Alexander
A1  - Peters, Marcell K.
A1  - Grimmer, Gudrun
A1  - Krischke, Beate
A1  - Geyer, Mareen
A1  - Schmitt, Thomas
A1  - Steffan‐Dewenter, Ingolf
T1  - Cryptic species and hidden ecological interactions of halictine bees along an elevational gradient
JF  - Ecology and Evolution
N2  - Changes of abiotic and biotic conditions along elevational gradients represent serious challenges to organisms which may promote the turnover of species, traits and biotic interaction partners. Here, we used molecular methods to study cuticular hydrocarbon (CHC) profiles, biotic interactions and phylogenetic relationships of halictid bees of the genus Lasioglossum along a 2,900 m elevational gradient at Mt. Kilimanjaro, Tanzania. We detected a strong species turnover of morphologically indistinguishable taxa with phylogenetically clustered cryptic species at high elevations, changes in CHC profiles, pollen resource diversity, and a turnover in the gut and body surface microbiome of bees. At high elevations, increased proportions of saturated compounds in CHC profiles indicate physiological adaptations to prevent desiccation. More specialized diets with higher proportions of low‐quality Asteraceae pollen imply constraints in the availability of food resources. Interactive effects of climatic conditions on gut and surface microbiomes, CHC profiles, and pollen diet suggest complex feedbacks among abiotic conditions, ecological interactions, physiological adaptations, and phylogenetic constraints as drivers of halictid bee communities at Mt. Kilimanjaro.
KW  - COI
KW  - cuticular chemistry
KW  - elevational gradient
KW  - Halictidae
KW  - microbiome metabarcoding
KW  - pollen metabarcoding
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-238853
VL  - 11
IS  - 12
SP  - 7700
EP  - 7712
ER  -