TY  - JOUR
A1  - Samper Agrelo, Iria
A1  - Schira-Heinen, Jessica
A1  - Beyer, Felix
A1  - Groh, Janos
A1  - Bütermann, Christine
A1  - Estrada, Veronica
A1  - Poschmann, Gereon
A1  - Bribian, Ana
A1  - Jadasz, Janusz J.
A1  - Lopez-Mascaraque, Laura
A1  - Kremer, David
A1  - Martini, Rudolf
A1  - Müller, Hans Werner
A1  - Hartung, Hans Peter
A1  - Adjaye, James
A1  - Stühler, Kai
A1  - Küry, Patrick
T1  - Secretome analysis of mesenchymal stem cell factors fostering oligodendroglial differentiation of neural stem cells in vivo
JF  - International Journal of Molecular Sciences
N2  - Mesenchymal stem cell (MSC)-secreted factors have been shown to significantly promote oligodendrogenesis from cultured primary adult neural stem cells (aNSCs) and oligodendroglial precursor cells (OPCs). Revealing underlying mechanisms of how aNSCs can be fostered to differentiate into a specific cell lineage could provide important insights for the establishment of novel neuroregenerative treatment approaches aiming at myelin repair. However, the nature of MSC-derived differentiation and maturation factors acting on the oligodendroglial lineage has not been identified thus far. In addition to missing information on active ingredients, the degree to which MSC-dependent lineage instruction is functional in vivo also remains to be established. We here demonstrate that MSC-derived factors can indeed stimulate oligodendrogenesis and myelin sheath generation of aNSCs transplanted into different rodent central nervous system (CNS) regions, and furthermore, we provide insights into the underlying mechanism on the basis of a comparative mass spectrometry secretome analysis. We identified a number of secreted proteins known to act on oligodendroglia lineage differentiation. Among them, the tissue inhibitor of metalloproteinase type 1 (TIMP-1) was revealed to be an active component of the MSC-conditioned medium, thus validating our chosen secretome approach.
KW  - neural stem cells
KW  - mesenchymal stem cells
KW  - transplantation
KW  - oligodendroglia
KW  - glial fate modulation
KW  - myelin
KW  - spinal cord
KW  - secretome
KW  - TIMP-1
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-285465
SN  - 1422-0067
VL  - 21
IS  - 12
ER  - 
TY  - JOUR
A1  - Temme, Sebastian
A1  - Friebe, Daniela
A1  - Schmidt, Timo
A1  - Poschmann, Gereon
A1  - Hesse, Julia
A1  - Steckel, Bodo
A1  - Stühler, Kai
A1  - Kunz, Meik
A1  - Dandekar, Thomas
A1  - Ding, Zhaoping
A1  - Akhyari, Payam
A1  - Lichtenberg, Artur
A1  - Schrader, Jürgen
T1  - Genetic profiling and surface proteome analysis of human atrial stromal cells and rat ventricular epicardium-derived cells reveals novel insights into their cardiogenic potential
JF  - Stem Cell Research
N2  - Epicardium-derived cells (EPDC) and atrial stromal cells (ASC) display cardio-regenerative potential, but the molecular details are still unexplored. Signals which induce activation, migration and differentiation of these cells are largely unknown. Here we have isolated rat ventricular EPDC and rat/human ASC and performed genetic and proteomic profiling. EPDC and ASC expressed epicardial/mesenchymal markers (WT-1, Tbx18, CD73,CD90, CD44, CD105), cardiac markers (Gata4, Tbx5, troponin T) and also contained phosphocreatine. We used cell surface biotinylation to isolate plasma membrane proteins of rEPDC and hASC, Nano-liquid chromatography with subsequent mass spectrometry and bioinformatics analysis identified 396 rat and 239 human plasma membrane proteins with 149 overlapping proteins. Functional GO-term analysis revealed several significantly enriched categories related to extracellular matrix (ECM), cell migration/differentiation, immunology or angiogenesis. We identified receptors for ephrin and growth factors (IGF, PDGF, EGF, anthrax toxin) known to be involved in cardiac repair and regeneration. Functional category enrichment identified clusters around integrins, PI3K/Akt-signaling and various cardiomyopathies. Our study indicates that EPDC and ASC have a similar molecular phenotype related to cardiac healing/regeneration. The cell surface proteome repository will help to further unravel the molecular details of their cardio-regenerative potential and their role in cardiac diseases.
KW  - Biology
KW  - Epicardium-derived cells
KW  - Human atrial stromal cells
KW  - Cell surface proteomics
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-172716
VL  - 25
ER  -