TY  - JOUR
A1  - Benhalevy, Daniel
A1  - Gupta, Sanjay K.
A1  - Danan, Charles H.
A1  - Ghosal, Suman
A1  - Sun, Hong-Wei
A1  - Kazemeier, Hinke G.
A1  - Paeschke, Katrin
A1  - Hafner, Markus
A1  - Juranek, Stefan A.
T1  - The Human CCHC-type Zinc Finger Nucleic Acid-Binding Protein Binds G-Rich Elements in Target mRNA Coding Sequences and Promotes Translation
JF  - Cell Reports
N2  - The CCHC-type zinc finger nucleic acid-binding protein (CNBP/ZNF9) is conserved in eukaryotes and is essential for embryonic development in mammals. It has been implicated in transcriptional, as well as post-transcriptional, gene regulation; however, its nucleic acid ligands and molecular function remain elusive. Here, we use multiple systems-wide approaches to identify CNBP targets and function. We used photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) to identify 8,420 CNBP binding sites on 4,178 mRNAs. CNBP preferentially bound G-rich elements in the target mRNA coding sequences, most of which were previously found to form G-quadruplex and other stable structures in vitro. Functional analyses, including RNA sequencing, ribosome profiling, and quantitative mass spectrometry, revealed that CNBP binding did not influence target mRNA abundance but rather increased their translational efficiency. Considering that CNBP binding prevented G-quadruplex structure formation in vitro, we hypothesize that CNBP is supporting translation by resolving stable structures on mRNAs.
KW  - PAR-CLIP
KW  - ribosome profiling
KW  - translational regulation
KW  - posttranscriptional gene regulation
KW  - zinc-finger
KW  - RNA binding protein
KW  - CLIP-seq
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-171122
VL  - 18
IS  - 12
ER  -