TY  - JOUR
A1  - Strauss, Armin
A1  - Moskalenko, Vasily
A1  - Tiurbe, Christian
A1  - Chodnevskaja, Irina
A1  - Timm, Stephan
A1  - Wiegering, Verena A.
A1  - Germer, Chrioph Thomas
A1  - Ulrichs, Karin
T1  - Goettingen Minipigs (GMP): Comparison of Two Different Models for Inducing Diabetes
N2  - Purpose: Preclinical experiments on large animals are indispensable for evaluating the effectiveness of diabetes therapies. Miniature swine are well suited for such studies due to their physiological and pathophysiological responses. Methods: We compare two methods for inducing diabetes in Goettingen minipigs (GMP), in five with the beta cell toxin streptozotocin (STZ) and in five other GMP by total pancreatectomy (PE). Glucose homeostasis was assessed with the intravenous glucose-tolerance test (IVGTT) and continual monitoring of interstitial glucose levels. At conclusion of the observation period, the pancreata were examined histologically. Three non-diabetic GMP served as control group. Results: The IVGTT revealed markedly diabetic profiles in both GMP groups. STZ-GMP were found to harbor residual C-peptides and scattered insulin-positive cells in the pancreas. PE-GMP survived the total pancreatectomy only with intensive postoperative care. Conclusions: Although both methods reliably induced diabetes in GMP, the PE-GMP clearly had more health problems and required a greater expenditure of time and resources. The PE-GMP model, however, was better at eliminating endogenous insulin and C-peptide than the STZ-GMP model.
KW  - Göttingen
KW  - Minischwein
KW  - diabetes
KW  - pig or swine
KW  - real-time glucose monitoring
KW  - intravenous glucose tolerance test
KW  - total pancreatectomy
KW  - streptozotocin
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-75119
ER  - 
TY  - CHAP
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Enzyme Kinetics of Commercial Collagenases and their Influence on Porcine Islet Isolation
N2  - No abstract available
KW  - Heterotransplantation
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45488
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Bosse, M.
A1  - Wacker, HH
A1  - Heiser, A.
A1  - Müller-Ruchholtz, W.
T1  - Histologic analysis of the porcine pancreas to improve islet yield and integrity after collagenase digestion
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45166
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Müller-Buchholtz, W.
T1  - Xenogeneic T-cell-mediated immune reactivity in the model of pig-to-humans: first findings with native stimulator cells
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44755
ER  - 
TY  - JOUR
A1  - Eckstein, V.
A1  - Müller-Buchholtz, W.
A1  - Ulrichs, Karin
T1  - Reaction patterns of natural xenophile antibodies in human sera with pancreatic islet cells and porcine lymphocytes
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44742
ER  - 
TY  - JOUR
A1  - Heiser, Axel
A1  - Ulrichs, Karin
A1  - Müller-Buchholtz, Wolfgang
T1  - Isolation of porcine pancreatic islets: low trypsin activity during the isolation procedure guarantees reproducible high islet yields
N2  - During the past few years, interest in xenotransplantation of porcine islets of Langerhans for the future therapy of type I diabetes has Increased markedly. Therefore, we established a semiautomated digestion method for isolating islets from the porcine pancreas. However, although the isolation technique was standardized and collagenase of controlled quality was used, we were unable to attain high islet yields with a satisfactory degree of reproducibility. One hypothesis was that varying degrees of interference by donor pancreatic enzymes were responsible for this failure. The aim of this stUdy was to examine the kinetics of four types of enzymatic activity during the isolation procedure, as well as their effects on islet yield: collagenase, trypsin, neutral protease, and clostripaln. Our results indicate that while exogenous collagenase activity decreases slightly during the isolation procedure, the activity of the pancreas enzymes neutral protease and trypsin increases. In some cases, trypsin activity increases very strongly. A strong increase in trypsin activity correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Addition of the protease inhibitor Pefabloc to the isolation medium results in low trypsin activity and reproducible high islet yields.
KW  - Langerhans-Inseln
KW  - islets of Langerhans
KW  - xenogeneic transplantation
KW  - swine
KW  - enzyme activation
KW  - enzyme inhibitors
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44719
ER  - 
TY  - JOUR
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Prophylactic trypsin inhibition during the isolation procedure guarantees reproducible, high porcine islet yields
N2  - Abstract: For isolating islets from the porcine pancreas, we established a semiautomated digestion method. Although the isolation technique was standardized and collagenase of controlled quality was used, until now the reproducibility of high islet yields was unsatisfactory. Our hypothesis was that pancreatic trypsin was responsible for this failure. The aim of this study was to investigate the effect of endogenous trypsin on islet yield. Our results demonstrate that a high trypsin level correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Specific inhibition of trypsin results in low trypsin activity and reproducible, high islet yields.
KW  - islets of Langerhans
KW  - xenogenic transplantation
KW  - swine
KW  - enzyme activation
KW  - enzyme inhibitors
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45531
ER  - 
TY  - CHAP
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Enzyme Activities in Commercial Collagenase Preparations and Their Kinetics During Islet Isolation Procedure
N2  - No abstract available
KW  - Transplantation
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45633
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Wang, H.
A1  - Müller-Buchholtz, W.
T1  - Down-regulation of xenophile antibodies by 15-deoxyspergualin in an experimental animal model
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44733
ER  - 
TY  - JOUR
A1  - Winoto-Morbach, S.
A1  - Krout, OS
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Lectin binding to acinar tissue for complete magnetophoretic purification of porcine pancreatic islets depends on the composition and pH of the incubation medium
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45183
ER  - 
TY  - JOUR
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Influence of porcine strain, age, and pH of the isolation medium on porcine pancreatic islet isolation success
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45171
ER  - 
TY  - JOUR
A1  - Heiser, A.
A1  - Müller-Ruchholtz, W.
A1  - Ulrichs, Karin
T1  - Isolation of Islets of Langerhans: Improvement of the Isolation Technique Using the Pig Model.
N2  - During the last view years, interest in pancreatic islet transplantation for the cure of type I diabetes has increased markedly. A serious barrier to clinical islet transplantation is the isolation of a sufficient mass of viable and functional islets. We used a porcine islet isolation model to examine various parameters of the isolation procedure and to improve isolation technique.
KW  - Langerhans-Inseln
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-87765
ER  - 
TY  - JOUR
A1  - Waaga, AM
A1  - Krzymanski, M.
A1  - Ulrichs, Karin
A1  - Wierusz-Wysocka, Bogna
A1  - Müller-Buchholtz, Wolfgang
T1  - Hematological effects of the new immunosuppressive drug 15-deoxyspergualin
N2  - Since systematic hematological studies on blood and bone marrow changes after treatment with 15-Deoxyspergualin (DOS) are lacking, a quantitative assessment was performed fourteen or twenty eight days after intraperitoneal application of DOS to rats. Further observations done 7 and 14 days after discontinuation of DOS administration allowed analysis of banc marrow regeneration. DOS induced lymphocytopenia, granUlocytopenia and anemia with a decrease of bone marrow cellularity due to suppression of cell maturation. The effect was dose-dependent and bone marrow as well as blood changes were observed in animals treated with doses from 0.5 to 10.0 mg/kg DOS. Within 14 days after termination of the treatment, rapid recovery with normalization of all hematological parameters was observed. In the light of our data, these hematological side effects may not be a major disadvantage, if DOS is used in doses below 2.5 mg/kg, and for a course of therapy which is limited to 7 to 14 days.
KW  - Chirurgie
KW  - 15-Deoxyspergualin
KW  - hematology
KW  - immunosuppression
KW  - bone marrow
KW  - regeneration
KW  - experimental therapy
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44701
ER  - 
TY  - JOUR
A1  - Breitkreuz, A.
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Müller-Ruchholz, W.
T1  - Long-term suppression of natural and graft-induced xenophile antibodies by short-term antigen-cyclophosphamide treatment
N2  - No abstract available
KW  - Chirurgie
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45262
ER  - 
TY  - CHAP
A1  - Hedke, K.
A1  - Eckstein, V.
A1  - May, G.
A1  - Kaden, J.
A1  - Müller-Ruchholtz, W.
A1  - Ulrichs, Karin
T1  - Production of Xenophile Antibodies (XA) in Type I Diabetic Patients is Strongly Influenced by Diabetes and/or Dialysis
N2  - No abstract available
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45642
ER  - 
TY  - CHAP
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Korsgren, O.
A1  - Müller-Ruchholtz, W.
T1  - Expression of Porcine Pancreatic Islet Antigens Varies and Determines Binding of Human Natural Xenophile Antibodies (NXA)
N2  - No abstract available
KW  - Immunbiologie
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45655
ER  - 
TY  - CHAP
A1  - Wang, HY
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Down-Regulation of Xenophile Antibodies by Specific Immunosuppressive Protocols to Facilitate Xenogeneic Organ Transplantation
N2  - No abstract available
KW  - Immunbiologie
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45669
ER  - 
TY  - CHAP
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Müller-Ruchholtz, W.
T1  - Xenogeneic Cellular Response of  Human Lymphocytes Against Porcine Lymphocytes and Isolated Pancreatic Islets
N2  - no abstract available
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45479
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Euler, HH
A1  - Schroeder, JO
A1  - Müller-Ruchhholtz, W.
T1  - Long-term specific suppression of antibody production by plasmapheresis and a short-term course of cyclophosphamide
N2  - No abstract available
KW  - Chirurgie
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45198
ER  - 
TY  - CHAP
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Müller-Ruchholtz, W.
T1  - The Human Cell Mediated Response to Xenogeneic (Porcine) Transplantation Antigen Depends on the Stimulator Cell Compartment
N2  - No abstract available
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45670
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Kaitschick, J.
A1  - Bartlett, R.
A1  - Müller-Ruchholtz, W.
T1  - Suppression of natural xenophile antibodies with the novel immunomodulating drug leflunomide
N2  - No abstract available
KW  - Chirurgie
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45202
ER  - 
TY  - JOUR
A1  - Krzymanski, Maciej
A1  - Waaga, Ana M.
A1  - Ulrichs, Karin
A1  - Deja, Aadam
A1  - Oko, Andrzej
A1  - Rommel, Thomas
A1  - Müller-Ruchholtz, Wolfgang
T1  - The influence of MHC class II antigen blockade by perfusion with a monoclonal antibody on rat renal graft survival
N2  - To decrease immunogenicity of the rat kidney, grafts were perfused with an anti-MHC class li monoclonal antibody (mAb ). How effectively this procedure blocked dass li-positive cells, which were mainly dendritic in appearance, was checked by immunostaining renal sections after perfusion and comparing them with in vitro stained sections. Optimum conditions were applied for graft pretreatment before transplantation. This procedure prolonged graft survival, though not satisfactorily from the biological point ofview (9.6 ± 0.8 versus 7.7 ± 0.5 days in the control group; P < 0.02). The dendritic cells were not killed but blocked. Several hours after transplantation, the mAb dissociated from these dass li-positive cells. It was also shown that donor cells migrate into the recipient's spieen early after transplantation. The number of these cells was smaller when the transplanted organ was perfused with the mAb. Further studies are suggested to deplete the graft of donor dendritic cells more adequately. They should also combine graft perfusion with antidass II mAb and recipient immunosuppression at reduced doses.
KW  - Chirurgie
KW  - Class II antigen blockade
KW  - rat
KW  - renal transplantation
KW  - Monoclonal antibody
KW  - dass II antigen blockade
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64431
ER  - 
TY  - CHAP
A1  - Eckstein, V.
A1  - Ulrichs, Karin
A1  - Meincke, G.
A1  - Mülller-Ruchholtz, W.
T1  - Transplantation of Diabetic Patients With Xenogeneic Pancreatic Islets Has to Consider Natural Xenophile Antibodies (NXA) In Patients Sera As a Major Obstacle to Success
N2  - No abstract available
KW  - Immunbiologie
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45707
ER  - 
TY  - CHAP
A1  - Winoto-Morbach, S.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - New Developments in Biodegradable Microspheres For Magnetic Separation Techniques
N2  - No abstract available
KW  - Immunbiologie
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45712
ER  - 
TY  - CHAP
A1  - Ulrichs, Karin
A1  - Euler, HH
A1  - Müller-Ruchholtz, W.
T1  - A Clinically Successful Protocol to Suppress Autoantibody Production in SLE Patients Is Analyzed For Its Efficacy To Inhibit Natural Xenophile Antibodies (NXA)
N2  - No abstract available
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45693
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, Wolfgang
T1  - Modulation of pancreatic islet allo immunogenicity and immunobiological in vitro and in vivo consequences
N2  - No abstract available.
KW  - Immunobiologie
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78261
ER  - 
TY  - JOUR
A1  - Krzymanski, M.
A1  - Waaga, A. M.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, Wolfgang
T1  - Long-standing rat kidney graft survival by a combination of organ perfusion with MHC class II monoclonal antibody and immunosuppression with reduced doses of 15-deoxyspergualin.
N2  - No abstract available
KW  - Niere
KW  - Ratte
KW  - MHC Klasse II
KW  - kidney
KW  - rat
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64442
ER  - 
TY  - JOUR
A1  - Waaga, AM
A1  - Ulrichs, Karin
A1  - Krzymanski, M.
A1  - Treumer, J.
A1  - Hansmann, ML
A1  - Rommel, T.
A1  - Müller-Ruchholz, W.
T1  - The immunosuppressive agent 15-deoxyspergualin induces tolerance and modulates MHC-antigen expression and interleukin-1 production in the early phase of rat allograft responses
N2  - No abstract available
KW  - Chirurgie
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45253
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Winoto-Morbach, S.
A1  - Hering, B.
A1  - Müller-Ruchholtz, W.
T1  - A Useful Biotechnological Approach to Solve the Problem of Graft Purity in Human Pancreatic Islet Transplantation
N2  - No abstract available
KW  - Chirurgie
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45619
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Mixed lymphocyte islet culture (MLIC) and its use in manipulation of human islet alloimmunogenicity
N2  - No abstract available
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45515
ER  - 
TY  - JOUR
A1  - Engemann, R.
A1  - Ulrichs, Karin
A1  - Thiede, A.
A1  - Müller-Ruchholtz, W.
A1  - Hamelmann, H.
T1  - Value of a physiological liver transplant model in rats. Induction of specific graft tolerance in a fully allogeneic strain combination
N2  - No abstract available
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45622
ER  - 
TY  - JOUR
A1  - Winoto-Morbach, S.
A1  - Ulrichs, Karin
A1  - Hering, BJ
A1  - Leyhausen, G.
A1  - Müller-Ruchholtz, W.
T1  - Lectins for electromagnetic purification of islets from humans and large mammals
N2  - No abstract available
KW  - Chirurgie
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45430
ER  - 
TY  - JOUR
A1  - Von Gaudecker, Brita
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, Wolfgang
T1  - Immunoelectron microscopic localization of MHC structures in isolated pancreatic rat islets
N2  - An immunogold-silver enhancement technique, which combines effective labeling of viable isolated islets with the ultrastructural resolution of cytological details, was applied in electron microscopy to identify major histocompatibility complex (MHC) structures on islet cells. Incubation of freshly isolated islets from CAP (RT1C) and LEW (RT1') rats with OX18, an MHC class I antibody, showed strong positive reactivity in macrophages and/or dendritic-like cells (M0-DCs) and vascular endothelial cells (VEs) and a comparatively weaker reactivity in endocrine a-, p-, and 8-ce"s. With MHC class" antibody OX6 (anti-I-A), M0-DCs were strongly labeled in both rat strains on the surface and on internal structures. Three of five particularly high titered batches of OX6 revealed MHC class" expression on VE and p-ce"s. Four days of in vitro culture in combination with a high concentration of glucose and interferon-'Y induced strong enhancement of MHC class I structures and, to a lesser extent, class " structures on p-ce"s.
KW  - Immunbiologie
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45596
ER  - 
TY  - GEN
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Identification and Manipulation of Human Islet Alloimmunogenicity: Morphologic and Functional in Vitro Studies with Various Islet Preparations
N2  - No abstract available
KW  - Immunbiologie
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45584
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Significant manipulative procedures to reduce immunogenicity of human islet allografts
N2  - No abstract available
KW  - Chirurgie
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45155
ER  - 
TY  - JOUR
A1  - Winoto-Morbach, S.
A1  - Leyhausen, G.
A1  - Schünke, M.
A1  - Ulrichs, Karin
A1  - Müller-Buchholtz, W.
T1  - Magnetic microspheres (MMS) coupled to selective lectins: a new tool for large-scale extraction and purification of human pancreatic islets
N2  - No abstract available
KW  - Chirurgie
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44789
ER  - 
TY  - JOUR
A1  - Winoto-Morbach, Supandi
A1  - Ulrichs, Karin
A1  - Leyhausen, Gaby
A1  - Müller-Ruchholtz, Wolfgang
T1  - New principle for large-scale preparation of purified human pancreas islets
N2  - Because successful human islet transplantation requires large quantities of viable islets that must be separated from the highly immunogenic exocrine tissue and because handpicking is too time-consuming and laborious to be clinically relevant, a new approach for solving this problem has been established in rat models. It is based on the principle that magnetic microspheres (MMSs) coupled to lectins with binding specificity for the exocrine tissue portion are trapped in an electromagnetic field, thus providing effluent islets of a high degree of purity. In this study our aim was to adapt this princip'le to human islet preparations. In this context our prime interest was focused on a lectin suitable for human pancreatic tissue. Of 19 different lectins tested, only 1, Wisteria floribunda agglutinin (WFA), is suitable, as shown by immunofluorescence, MMS-Iectin binding, and magnetic separation
KW  - Immunbiologie
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45600
ER  - 
TY  - JOUR
A1  - Kekow, Jörn
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, Wolfgang
A1  - Gross, Wolfgang L.
T1  - Measurement of rat insulin. Enzyme-linked immunosorbent assay with increased sensitivity, high accuracy, and greater practicability than established radioimmunoassay
N2  - Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IR! measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELlSA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle of competitive saturation. In this ELlSA, IRI can be determined in a wide range of concentrations, corresponding to the standards. The standard curve ranges from 100 to 0.049 ng/mllRI (1 ng/ml - 23.4 JLU/ml - 172 pM rat insulin). The statistical analysis shows between- and within-assay coefficients of variation of :515%. Diabetes 37:321-26,1988
KW  - Chirurgie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45543
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Expression of MHC Structures on Immunologically Reactive and Non Reactive Cells in Islets of Langerhans and Other Tissues
N2  - No abstract available
KW  - Chirurgie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45557
ER  - 
TY  - JOUR
A1  - Winoto-Morbach, S.
A1  - Leyhausen, G.
A1  - Müller-Ruchholtz, W.
A1  - Ulrichs, Karin
T1  - The Electromagnetic Separation Principle: A Basis for Human Pancreatic Islet Transplantation
N2  - No abstract available
KW  - Allergie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-82520
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Nöthling, R.
A1  - Keller, R.
A1  - Heusermann, U.
A1  - Müller-Buchholtz, W.
T1  - Genetically determined variation of constitutive major histocompatibility complex class II antigen expression in various rat strains and cell types
N2  - No abstract available
KW  - Chirurgie
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44769
ER  - 
TY  - JOUR
A1  - Müller-Buchholtz, W.
A1  - Leyhausen, G.
A1  - Peterson, P.
A1  - Schubert, G.
A1  - Ulrichs, Karin
T1  - A simple methodological principle for large scale extraction and purification of collagenase-digested islets
N2  - No abstract available
KW  - Chirurgie
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44770
ER  - 
TY  - JOUR
A1  - Kekow, J.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
A1  - Gross, WL
T1  - Pancreas transplantation: a study of insulin secretion in isolated islets of Langerhans and in sera using a new enzyme-linked immunosorbent assay
N2  - No abstract available
KW  - Chirurgie
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45229
ER  - 
TY  - JOUR
A1  - Falk, W.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - 15-Deoxyspergualin (a new guanidine-like drug) blocks T lymphocyte proliferation
N2  - No abstract available
KW  - Chirurgie
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45215
ER  - 
TY  - JOUR
A1  - Dickneite, G.
A1  - Schorlemmer, H. U.
A1  - Sedlacek, H. H.
A1  - Falk, W.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Suppression of macrophage function and prolongation of graft survival by the new guanidinic-like structure, 15-deoxyspergualin
N2  - No abstract available.
KW  - Makrophage
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86991
ER  - 
TY  - JOUR
A1  - Laus, R.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Molecular Specificity of Human Heterophile Antibodies (HHA) in Normal Human Serum (NHS) Reacting with Xenogeneic Cells.
N2  - No abstract available
KW  - Immunobiologie
Y1  - 1986
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72921
ER  - 
TY  - JOUR
A1  - Kekow, J.
A1  - Ulrichs, Karin
T1  - Determination of total immunoreactive rat insulin (IRI) in culture supernatants of rat islets by an enzyme linked immunosorbent assay (ELISA) as a routine method to assess the viability of rat islets prior to their use in islet transplantation
N2  - No abstract available
KW  - Immunobiologie
Y1  - 1986
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72989
ER  - 
TY  - JOUR
A1  - Deltz, Eberhard
A1  - Ulrichs, Karin
A1  - Schack, Thorsten
A1  - Friedrichs, Birgit
A1  - Müller-Ruchholtz, Wolfgang
A1  - Müller-Hermelink, Hans K.
A1  - Thiede, Arnulf
T1  - Graft-versus-host reaction in small bowel transplantation and possibilities for its circumvention
N2  - No abstract available
KW  - Dünndarm
KW  - Transplantation
KW  - transplantation
KW  - small bowel
Y1  - 1986
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64425
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Keller, R.
A1  - Müller-Ruchholtz, W.
T1  - Serological demonstration and manipulation of passenger cells (PC) in pancreas islet grafts
N2  - No abstract available
KW  - Immunobiologie
Y1  - 1985
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72944
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Further Analyses of Pancreas Islet Immunogenicity, a Major Barrier to Successful Islet Transplantation
N2  - No abstract available
KW  - Chirurgie
Y1  - 1985
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45563
ER  -