TY  - JOUR
A1  - Gorboulev, Valentin
A1  - Büchner, Hubert
A1  - Akhundova, Aida
A1  - Fahrenholz, Falk
T1  - Molecular cloning and functional characterization of V<sub>2</sub> [8-Iysine] vasopressin and oxytocin receptors from a pig kidney cell line (LLC-PK1)
N2  - No abstract available
KW  - Biologie
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59311
ER  - 
TY  - JOUR
A1  - Gorboulev, Valentin
A1  - Akhundova, Aida
A1  - Büchner, Hubert
A1  - Fahrenholz, Falk
T1  - Molecular cloning of a new bombesin receptor subtype expressed in uterus during pregnancy
N2  - The homology screening approach has been used to clone a new member of the guanine-nucleotidebinding-protein-coupled receptor superfamily from guinea pig uterus. The cloned cDNA encodes a 399-amino-acid protein and shows the highest amino acid similarity to members of the bombesin receptor family; 52% and 47% similarity to the gastrin-releasing-peptide (GRP) receptor and the neuromedin-B receptor, respectively. Bindingexperiments with the stably transfected LLC-PK<sub>1</sub> cell line expressing the new receptor protein confmned the bombesin-like nature of the cloned receptor. The relative order ofligand affinity, GRP = neuromedin C >> neuromedin B, suggests that the cloned cDNA represents the GRP subtype rather than the neuromedin-B subtype of bombesin receptors. Northern-blot analysis of mRNA species from several guinea-pig tissues showed that the mRNA for the new bombesin receptor subtype is expressed mainly in uteri of pregnant animals.
KW  - Biologie
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59304
ER  -