TY  - JOUR
A1  - Ott, Christine
A1  - Dorsch, Eva
A1  - Fraunholz, Martin
A1  - Straub, Sebastian
A1  - Kozjak-Pavlovic, Vera
T1  - Detailed Analysis of the Human Mitochondrial Contact Site Complex Indicate a Hierarchy of Subunits
JF  - PLoS One
N2  - Mitochondrial inner membrane folds into cristae, which significantly increase its surface and are important for mitochondrial function. The stability of cristae depends on the mitochondrial contact site (MICOS) complex. In human mitochondria, the inner membrane MICOS complex interacts with the outer membrane sorting and assembly machinery (SAM) complex, to form the mitochondrial intermembrane space bridging complex (MIB). We have created knockdown cell lines of most of the MICOS and MIB components and have used them to study the importance of the individual subunits for the cristae formation and complex stability. We show that the most important subunits of the MIB complex in human mitochondria are Mic60/Mitofilin, Mic19/CHCHD3 and an outer membrane component Sam50. We provide additional proof that ApoO indeed is a subunit of the MICOS and MIB complexes and propose the name Mic23 for this protein. According to our results, Mic25/CHCHD6, Mic27/ApoOL and Mic23/ApoO appear to be periphery subunits of the MICOS complex, because their depletion does not affect cristae morphology or stability of other components.
KW  - co-immunoprecipitation
KW  - motor proteins
KW  - mitochondria
KW  - membrane potential
KW  - membrane proteins
KW  - protein complexes
KW  - mitochondrial membrane
KW  - outer membrane proteins
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-125347
VL  - 10
IS  - 3
ER  -