TY - JOUR A1 - Deltz, Eberhard A1 - Ulrichs, Karin A1 - Schack, Thorsten A1 - Friedrichs, Birgit A1 - Müller-Ruchholtz, Wolfgang A1 - Müller-Hermelink, Hans K. A1 - Thiede, Arnulf T1 - Graft-versus-host reaction in small bowel transplantation and possibilities for its circumvention N2 - No abstract available KW - Dünndarm KW - Transplantation KW - transplantation KW - small bowel Y1 - 1986 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64425 ER - TY - JOUR A1 - Kekow, Jörn A1 - Ulrichs, Karin A1 - Müller-Ruchholtz, Wolfgang A1 - Gross, Wolfgang L. T1 - Measurement of rat insulin. Enzyme-linked immunosorbent assay with increased sensitivity, high accuracy, and greater practicability than established radioimmunoassay N2 - Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IR! measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELlSA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle of competitive saturation. In this ELlSA, IRI can be determined in a wide range of concentrations, corresponding to the standards. The standard curve ranges from 100 to 0.049 ng/mllRI (1 ng/ml - 23.4 JLU/ml - 172 pM rat insulin). The statistical analysis shows between- and within-assay coefficients of variation of :515%. Diabetes 37:321-26,1988 KW - Chirurgie Y1 - 1988 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45543 ER - TY - JOUR A1 - Krzymanski, M. A1 - Waaga, A. M. A1 - Ulrichs, Karin A1 - Müller-Ruchholtz, Wolfgang T1 - Long-standing rat kidney graft survival by a combination of organ perfusion with MHC class II monoclonal antibody and immunosuppression with reduced doses of 15-deoxyspergualin. N2 - No abstract available KW - Niere KW - Ratte KW - MHC Klasse II KW - kidney KW - rat Y1 - 1991 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64442 ER - TY - JOUR A1 - Krzymanski, Maciej A1 - Waaga, Ana M. A1 - Ulrichs, Karin A1 - Deja, Aadam A1 - Oko, Andrzej A1 - Rommel, Thomas A1 - Müller-Ruchholtz, Wolfgang T1 - The influence of MHC class II antigen blockade by perfusion with a monoclonal antibody on rat renal graft survival N2 - To decrease immunogenicity of the rat kidney, grafts were perfused with an anti-MHC class li monoclonal antibody (mAb ). How effectively this procedure blocked dass li-positive cells, which were mainly dendritic in appearance, was checked by immunostaining renal sections after perfusion and comparing them with in vitro stained sections. Optimum conditions were applied for graft pretreatment before transplantation. This procedure prolonged graft survival, though not satisfactorily from the biological point ofview (9.6 ± 0.8 versus 7.7 ± 0.5 days in the control group; P < 0.02). The dendritic cells were not killed but blocked. Several hours after transplantation, the mAb dissociated from these dass li-positive cells. It was also shown that donor cells migrate into the recipient's spieen early after transplantation. The number of these cells was smaller when the transplanted organ was perfused with the mAb. Further studies are suggested to deplete the graft of donor dendritic cells more adequately. They should also combine graft perfusion with antidass II mAb and recipient immunosuppression at reduced doses. KW - Chirurgie KW - Class II antigen blockade KW - rat KW - renal transplantation KW - Monoclonal antibody KW - dass II antigen blockade Y1 - 1991 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64431 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Müller-Ruchholtz, Wolfgang T1 - Modulation of pancreatic islet allo immunogenicity and immunobiological in vitro and in vivo consequences N2 - No abstract available. KW - Immunobiologie Y1 - 1991 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78261 ER - TY - JOUR A1 - Von Gaudecker, Brita A1 - Ulrichs, Karin A1 - Müller-Ruchholtz, Wolfgang T1 - Immunoelectron microscopic localization of MHC structures in isolated pancreatic rat islets N2 - An immunogold-silver enhancement technique, which combines effective labeling of viable isolated islets with the ultrastructural resolution of cytological details, was applied in electron microscopy to identify major histocompatibility complex (MHC) structures on islet cells. Incubation of freshly isolated islets from CAP (RT1C) and LEW (RT1') rats with OX18, an MHC class I antibody, showed strong positive reactivity in macrophages and/or dendritic-like cells (M0-DCs) and vascular endothelial cells (VEs) and a comparatively weaker reactivity in endocrine a-, p-, and 8-ce"s. With MHC class" antibody OX6 (anti-I-A), M0-DCs were strongly labeled in both rat strains on the surface and on internal structures. Three of five particularly high titered batches of OX6 revealed MHC class" expression on VE and p-ce"s. Four days of in vitro culture in combination with a high concentration of glucose and interferon-'Y induced strong enhancement of MHC class I structures and, to a lesser extent, class " structures on p-ce"s. KW - Immunbiologie Y1 - 1989 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45596 ER - TY - JOUR A1 - Winoto-Morbach, Supandi A1 - Ulrichs, Karin A1 - Leyhausen, Gaby A1 - Müller-Ruchholtz, Wolfgang T1 - New principle for large-scale preparation of purified human pancreas islets N2 - Because successful human islet transplantation requires large quantities of viable islets that must be separated from the highly immunogenic exocrine tissue and because handpicking is too time-consuming and laborious to be clinically relevant, a new approach for solving this problem has been established in rat models. It is based on the principle that magnetic microspheres (MMSs) coupled to lectins with binding specificity for the exocrine tissue portion are trapped in an electromagnetic field, thus providing effluent islets of a high degree of purity. In this study our aim was to adapt this princip'le to human islet preparations. In this context our prime interest was focused on a lectin suitable for human pancreatic tissue. Of 19 different lectins tested, only 1, Wisteria floribunda agglutinin (WFA), is suitable, as shown by immunofluorescence, MMS-Iectin binding, and magnetic separation KW - Immunbiologie Y1 - 1989 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45600 ER -