TY  - JOUR
A1  - Spring, Herbert
A1  - Trendelenbrug, Michael F.
A1  - Scheer, Ulrich
A1  - Franke, Werner W.
A1  - Herth, Werner
T1  - Structural and biochemical studies of the primary nucleus of two green algal species, Acetabularia mediterranea and Acetabularia major
N2  - Primary (giant) nuclei of the green algae Acetabularia mediterranea and A. major were studied by light and electron microscopy using in situ fixed material as well as manually isolated nuclear components. In addition, cytochemical reactions of nuclear structures and biochemical determinations of nuclear and cytoplasmic RNA and of genome DNA content were performed. The data obtained and the structures observed are interpreted as demonstralions of transcriptional activities of different gene classes. The most prominent class is the nucleolar cistrons of precursors of ribosomal RNA which occur highly repeated in clusters in the form of regularly alternating intercepts on deoxyribonucleoprotein axes of transcribed rDNA, the fibril-covered matrix units, and the fibril-free "spacer" segments. A description and a classification of the various structural complexes which seem to represent transcriptional activities is given. Quantitative evaluations of these arrangements are presented. The morphology and the dimensions of such structures are compared with the RNA molecular weight determinations and with the corresponding data reported from various animal cell systems. It is suggested that the formation of the giant nucleus is correlated with, and probably due to, an enormous amplification of transcriptionally active rDNA and packing of the extrachromosomal copies into the large nucleolar aggregate bodies.
KW  - Cytologie
KW  - Nucleolus
KW  - electron microscopy
KW  - Acetabularia
KW  - transcription
KW  - gene activity
KW  - ribosomes
Y1  - 1974
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-40600
ER  - 
TY  - JOUR
A1  - Weber, Klaus
A1  - Osborn, Mary
A1  - Franke, Werner W.
A1  - Seib, Erinita
A1  - Scheer, Ulrich
A1  - Herth, Werner
T1  - Identification of microtubular structures in diverse plant and animal cells by immunological cross-reaction revealed in immunofluorescence microscopy using antibodies against tubulin from porcine brain
N2  - Antibody against tubulin from porcine brain was used to evaluate the immunological cross reactivity of tubulin from a variety of animal and plant cells. Indirect immunofluorescence microscopy revealed microtubule-containing structures including cytoplasmic microtubules, spindle microtubules, cilia and fIagella. Thus tubulin from diverse species of both mammals and plants show immunological cross-reactivity with tubulin from porcine brain. Results obtained by immunofluorescence microscopy are whenever possible compared with previously known ultrastructural results obtained by electron microscopy.
KW  - Cytologie
KW  - Microtubules
KW  - immunofluorescence
KW  - evolution
KW  - antibody
KW  - sperm
Y1  - 1977
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-41383
ER  - 
TY  - JOUR
A1  - Franke, Werner W.
A1  - Scheer, Ulrich
A1  - Herth, Werner
T1  - Cytology, general and molecular cytology
N2  - The present article had originally been conceived as a review on endomembranes, the plasma membrane, and the major product of membrane-bound activities, the cell wall material. However, limitations of space and the cascading number of pertinent literature articles made it necessary to confine this to one group of membranes and one type of cell wall components. Therefore, we shall begin our survey on the biochemical and cytological aspects of membranes by a review of the class of the pore complex bearing endomembranes, i.e. the nuclear envelope and the annulate lamellae (AL). Next year the membranes of the endoplasmic reticulum and the dictyosomes will be dealt with in conjunction with a discussion of the various intracellular vesicles, the tonoplast and the plasmalemma.
KW  - Botanik
Y1  - 1974
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-39499
ER  - 
TY  - JOUR
A1  - Franke, Werner W.
A1  - Jarasch, Ernst-Dieter
A1  - Herth, Werner
A1  - Scheer, Ulrich
A1  - Zerban, Heide
T1  - Cytology : general and molecular cytology
N2  - The present review discusses some general aspects of membrane structure and problems of membrane isolation and membrane biochemistry, with particular focus on the endoplasmic reticulum.
KW  - Botanik
Y1  - 1975
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-41458
ER  - 
TY  - JOUR
A1  - Franke, Werner W.
A1  - Scheer, Ulrich
A1  - Herth, Werner
T1  - Cytologie, allgemeine und molekulare Cytologie
N2  - No abstract available
Y1  - 1973
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-40547
ER  -