TY  - JOUR
A1  - Lichter, Katharina
A1  - Paul, Mila Marie
A1  - Pauli, Martin
A1  - Schoch, Susanne
A1  - Kollmannsberger, Philip
A1  - Stigloher, Christian
A1  - Heckmann, Manfred
A1  - Sirén, Anna-Leena
T1  - Ultrastructural analysis of wild-type and RIM1α knockout active zones in a large cortical synapse
JF  - Cell Reports
N2  - Rab3A-interacting molecule (RIM) is crucial for fast Ca\(^{2+}\)-triggered synaptic vesicle (SV) release in presynaptic active zones (AZs). We investigated hippocampal giant mossy fiber bouton (MFB) AZ architecture in 3D using electron tomography of rapid cryo-immobilized acute brain slices in RIM1α\(^{−/−}\) and wild-type mice. In RIM1α\(^{−/−}\), AZs are larger with increased synaptic cleft widths and a 3-fold reduced number of tightly docked SVs (0–2 nm). The distance of tightly docked SVs to the AZ center is increased from 110 to 195 nm, and the width of their electron-dense material between outer SV membrane and AZ membrane is reduced. Furthermore, the SV pool in RIM1α\(^{−/−}\) is more heterogeneous. Thus, RIM1α, besides its role in tight SV docking, is crucial for synaptic architecture and vesicle pool organization in MFBs.
KW  - active zone
KW  - acute brain slices
KW  - CA3
KW  - electron tomography
KW  - high-pressure freezing
KW  - hippocampal mossy fiber bouton
KW  - RIM1α
KW  - SV pool
KW  - synaptic ultrastructure
KW  - presynaptic
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-300913
VL  - 40
IS  - 12
ER  -