TY  - JOUR
A1  - Steinmetzger, Christian
A1  - Bäuerlein, Carmen
A1  - Höbartner, Claudia
T1  - Supramolecular fluorescence resonance energy transfer in nucleobase-modified fluorogenic RNA aptamers
JF  - Angewandte Chemie, International Edition
N2  - RNA aptamers form compact tertiary structures and bind their ligands in specific binding sites. Fluorescence-based strategies reveal information on structure and dynamics of RNA aptamers. Here we report the incorporation of the universal emissive nucleobase analog 4-cyanoindole into the fluorogenic RNA aptamer Chili, and its application as a donor for supramolecular FRET to bound ligands DMHBI+ or DMHBO+. The photophysical properties of the new nucleobase-ligand-FRET pair revealed structural restraints for the overall RNA aptamer organization and identified nucleotide positions suitable for FRET-based readout of ligand binding. This strategy is generally suitable for binding site mapping and may also be applied for responsive aptamer devices.
KW  - RNA aptamers
KW  - fluorescence resonance energy transfer
KW  - large stokes shift
KW  - isomorphic nucleobase analog
KW  - structure probing
KW  - structure probes
KW  - stokes shift
KW  - Fluoreszenzresonanz-Energietransfer
KW  - Isomorphe Nukleobasen-Analoga
KW  - RNA-Aptamere
KW  - Stokes-Verschiebung
KW  - Struktursonden
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-203084
N1  - Parallel erschienen in Angewandte Chemie 2020,132, 6826–6830. DOI: 10.1002/ange.201916707 (Deutsche Ausgabe).
VL  - 59
ER  -