TY  - JOUR
A1  - Stepula, Elzbieta
A1  - König, Matthias
A1  - Wang, Xin‐Ping
A1  - Levermann, Janina
A1  - Schimming, Tobias
A1  - Kasimir‐Bauer, Sabine
A1  - Schilling, Bastian
A1  - Schlücker, Sebastian
T1  - Localization of PD‐L1 on single cancer cells by iSERS microscopy with Au/Au core/satellite nanoparticles
JF  - Journal of Biophotonics
N2  - Programmed cell death‐ligand 1 (PD‐L1) is an important predictive biomarker. The detection of PD‐L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno‐SERS microscopy (iSERS) for localizing PD‐L1 on single cancer SkBr‐3 cells. A central advantage of iSERS is that the disturbing autofluorescence from cells and tissues can be efficiently minimized by red to near‐infrared laser excitation. In this study we employed Au/Au core/satellite nanoparticles as SERS nanotags because of their remarkable signal brightness and colloidal stability upon red laser excitation. False‐color iSERS images of the positive and negative controls clearly reveal the specific localization of PD‐L1 with SERS nanotag‐labeled antibodies.
KW  - gold nanoparticles
KW  - PD‐L1
KW  - Raman
KW  - SERS
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-212655
VL  - 13
IS  - 3
ER  -