TY  - JOUR
A1  - Jauch, A.
A1  - Lutz, Werner K.
T1  - In vivo assay for somatic point mutations induced by genotoxic carcinogens: incorporation of [\(^{35}\)S]methionine into a rat liver cytochrome b\(_5\) normally lacking sulphur-containing amino acids
N2  - The trypsin fragments of rat liver microsomal cytochron1e b\(_5\) (Tb\(_5\)) lack both methionine (met) and cysteine (cys), i.e., the sulphur-containing antino acids. Tb\(_5\) should therefore contain no 358-radioactivity after isolation from animals treated wHh [\(^{35}\)S]met or [\(^{36}\)S]cys. If, however, the nucleic acids coding for this polypeptide have been damaged by a genotoxic carcinogen, a miscoding could result in an incorporation of met or cys into the polypeptide so that Tb\(_8\) could now be \(^{36}\)S-radiolabelled. Two experiments are descrihed. the first one where a toxic regimen of N -nitrosomorpholine (NNM) to rats resulted in a significant increase of \(^{35}\)S-radioactivity in the Tbs of liver microsomes, and a second experiment with a non-toxic regimen of N,N diethylnitrosamine (DENA), where no increase was observable.
KW  - Toxikologie
KW  - MammaJian mutagenicity test
KW  - Pointmutation
KW  - Protein coding
KW  - Cytochrome b5
KW  - Amino acid composition
KW  - Rat Iiver microsomes
Y1  - 1983
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61047
ER  -