TY  - JOUR
A1  - Winoto-Morbach, S.
A1  - Ulrichs, Karin
A1  - Hering, BJ
A1  - Leyhausen, G.
A1  - Müller-Ruchholtz, W.
T1  - Lectins for electromagnetic purification of islets from humans and large mammals
N2  - No abstract available
KW  - Chirurgie
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45430
ER  - 
TY  - JOUR
A1  - Winoto-Morbach, S.
A1  - Leyhausen, G.
A1  - Schünke, M.
A1  - Ulrichs, Karin
A1  - Müller-Buchholtz, W.
T1  - Magnetic microspheres (MMS) coupled to selective lectins: a new tool for large-scale extraction and purification of human pancreatic islets
N2  - No abstract available
KW  - Chirurgie
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44789
ER  - 
TY  - JOUR
A1  - Winoto-Morbach, S.
A1  - Krout, OS
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Lectin binding to acinar tissue for complete magnetophoretic purification of porcine pancreatic islets depends on the composition and pH of the incubation medium
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45183
ER  - 
TY  - JOUR
A1  - Wiegering, Armin
A1  - Korb, Doreen
A1  - Thalheimer, Andreas
A1  - Kämmerer, Ulrike
A1  - Allmanritter, Jan
A1  - Matthes, Niels
A1  - Linnebacher, Michael
A1  - Schlegel, Nicolas
A1  - Klein, Ingo
A1  - Ergün, Süleyman
A1  - Germer, Christoph-Thomas
A1  - Otto, Christoph
T1  - E7080 (Lenvatinib), a Multi-Targeted Tyrosine Kinase Inhibitor, Demonstrates Antitumor Activities Against Colorectal Cancer Xenografts
N2  - Clinical prognosis of metastasized colorectal carcinoma (CRC) is still not at desired levels and novel drugs are needed. Here, we focused on the multi-tyrosine kinase inhibitor E7080 (Lenvatinib) and assessed its therapeutic efficacy against human CRC cell lines in vitro and human CRC xenografts in vivo. The effect of E7080 on cell viability was examined on 10 humanCRCcell lines and humanendothelial cells (HUVEC). The inhibitory effect of E7080 on VEGF-induced angiogenesis was studied in an ex vivo mouse aortic ring angiogenesis assay. In addition, the efficacy of E7080 against xenografts derived fromCRC cell lines and CRC patient resection specimenswithmutated KRASwas investigated in vivo. Arelatively low cytotoxic effect of E7080 on CRC cell viabilitywas observed in vitro. Endothelial cells (HUVEC)weremore susceptible to the incubation with E7080. This is in line with the observation that E7080 demonstrated an anti-angiogenic effect in a three-dimensional ex vivo mouse aortic ring angiogenesis assay. E7080 effectively disrupted CRC cell-mediated VEGF-stimulated growth of HUVEC in vitro. Daily in vivo treatment with E7080 (5 mg/kg) significantly delayed the growth of KRAS mutated CRC xenografts with decreased density of tumor-associated vessel formations and without tumor regression. This observation is in line with results that E7080 did not significantly reduce the number of Ki67-positive cells in CRC xenografts. The results suggest antiangiogenic activity of E7080 at a dosage thatwas well tolerated by nudemice. E7080 may provide therapeutic benefits in the treatment of CRC with mutated KRAS.
KW  - Chirurgie
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-111165
ER  - 
TY  - JOUR
A1  - Waaga, AM
A1  - Ulrichs, Karin
A1  - Krzymanski, M.
A1  - Treumer, J.
A1  - Hansmann, ML
A1  - Rommel, T.
A1  - Müller-Ruchholz, W.
T1  - The immunosuppressive agent 15-deoxyspergualin induces tolerance and modulates MHC-antigen expression and interleukin-1 production in the early phase of rat allograft responses
N2  - No abstract available
KW  - Chirurgie
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45253
ER  - 
TY  - JOUR
A1  - Waaga, AM
A1  - Krzymanski, M.
A1  - Ulrichs, Karin
A1  - Wierusz-Wysocka, Bogna
A1  - Müller-Buchholtz, Wolfgang
T1  - Hematological effects of the new immunosuppressive drug 15-deoxyspergualin
N2  - Since systematic hematological studies on blood and bone marrow changes after treatment with 15-Deoxyspergualin (DOS) are lacking, a quantitative assessment was performed fourteen or twenty eight days after intraperitoneal application of DOS to rats. Further observations done 7 and 14 days after discontinuation of DOS administration allowed analysis of banc marrow regeneration. DOS induced lymphocytopenia, granUlocytopenia and anemia with a decrease of bone marrow cellularity due to suppression of cell maturation. The effect was dose-dependent and bone marrow as well as blood changes were observed in animals treated with doses from 0.5 to 10.0 mg/kg DOS. Within 14 days after termination of the treatment, rapid recovery with normalization of all hematological parameters was observed. In the light of our data, these hematological side effects may not be a major disadvantage, if DOS is used in doses below 2.5 mg/kg, and for a course of therapy which is limited to 7 to 14 days.
KW  - Chirurgie
KW  - 15-Deoxyspergualin
KW  - hematology
KW  - immunosuppression
KW  - bone marrow
KW  - regeneration
KW  - experimental therapy
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44701
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Winoto-Morbach, S.
A1  - Hering, B.
A1  - Müller-Ruchholtz, W.
T1  - A Useful Biotechnological Approach to Solve the Problem of Graft Purity in Human Pancreatic Islet Transplantation
N2  - No abstract available
KW  - Chirurgie
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45619
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Wang, H.
A1  - Müller-Buchholtz, W.
T1  - Down-regulation of xenophile antibodies by 15-deoxyspergualin in an experimental animal model
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44733
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Nöthling, R.
A1  - Keller, R.
A1  - Heusermann, U.
A1  - Müller-Buchholtz, W.
T1  - Genetically determined variation of constitutive major histocompatibility complex class II antigen expression in various rat strains and cell types
N2  - No abstract available
KW  - Chirurgie
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44769
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Expression of MHC Structures on Immunologically Reactive and Non Reactive Cells in Islets of Langerhans and Other Tissues
N2  - No abstract available
KW  - Chirurgie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45557
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Further Analyses of Pancreas Islet Immunogenicity, a Major Barrier to Successful Islet Transplantation
N2  - No abstract available
KW  - Chirurgie
Y1  - 1985
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45563
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Significant manipulative procedures to reduce immunogenicity of human islet allografts
N2  - No abstract available
KW  - Chirurgie
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45155
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Kaitschick, J.
A1  - Bartlett, R.
A1  - Müller-Ruchholtz, W.
T1  - Suppression of natural xenophile antibodies with the novel immunomodulating drug leflunomide
N2  - No abstract available
KW  - Chirurgie
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45202
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Euler, HH
A1  - Schroeder, JO
A1  - Müller-Ruchhholtz, W.
T1  - Long-term specific suppression of antibody production by plasmapheresis and a short-term course of cyclophosphamide
N2  - No abstract available
KW  - Chirurgie
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45198
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Müller-Buchholtz, W.
T1  - Xenogeneic T-cell-mediated immune reactivity in the model of pig-to-humans: first findings with native stimulator cells
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44755
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Bosse, M.
A1  - Wacker, HH
A1  - Heiser, A.
A1  - Müller-Ruchholtz, W.
T1  - Histologic analysis of the porcine pancreas to improve islet yield and integrity after collagenase digestion
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45166
ER  - 
TY  - JOUR
A1  - Müller-Buchholtz, W.
A1  - Leyhausen, G.
A1  - Peterson, P.
A1  - Schubert, G.
A1  - Ulrichs, Karin
T1  - A simple methodological principle for large scale extraction and purification of collagenase-digested islets
N2  - No abstract available
KW  - Chirurgie
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44770
ER  - 
TY  - JOUR
A1  - Laus, R.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Carbohydrate-specific human heterophile antibodies in normal human sera that react with xenogeneic cells
N2  - Human heterophile antibodies (HHA) that are present in normal human sera (NHS)play an important role in hyperacute xenograft rejection. The aim of this study was to analyze the occurrence, mode of action and molecular specificity of HHA in NHS that are directed against xenogeneic Iymphocytes (isolated from mouse, rat, guinea pig, rabbit, cattle and pig) and isolated rat pancreatic islets. All sera contained variable amounts of HHA that killed the target cells via the classical complement pathway. The cytotoxic activity of these HHA was specifically inhibited by certain carbohydrates (a-D-melibiose, ß-Iactose, ß-gentiobiose, ß-cellobiose, D-mannose, N-acetyl-ß-D-mannosamine and a-D-rhamnose) and by rat IgM. By means of affinity chromatography with immobilized inhibitors we obtained an antibody preparation of mainly IgG type from NHS (up to 3.5 mg/IO ml serum) that reacted strongly with rat lymphocytes and isolated rat pancreatic islets. Though thus far residual xenospecific antibody activity has remained in the sera even after multiple affinity chromatography, these data suggest that specific elimination of HHA is feasible and that it may be thus possible to overcome a major obstacle to xenotransplantation.
KW  - Immunobiologie
KW  - Chirurgie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78271
ER  - 
TY  - JOUR
A1  - Krzymanski, Maciej
A1  - Waaga, Ana M.
A1  - Ulrichs, Karin
A1  - Deja, Aadam
A1  - Oko, Andrzej
A1  - Rommel, Thomas
A1  - Müller-Ruchholtz, Wolfgang
T1  - The influence of MHC class II antigen blockade by perfusion with a monoclonal antibody on rat renal graft survival
N2  - To decrease immunogenicity of the rat kidney, grafts were perfused with an anti-MHC class li monoclonal antibody (mAb ). How effectively this procedure blocked dass li-positive cells, which were mainly dendritic in appearance, was checked by immunostaining renal sections after perfusion and comparing them with in vitro stained sections. Optimum conditions were applied for graft pretreatment before transplantation. This procedure prolonged graft survival, though not satisfactorily from the biological point ofview (9.6 ± 0.8 versus 7.7 ± 0.5 days in the control group; P < 0.02). The dendritic cells were not killed but blocked. Several hours after transplantation, the mAb dissociated from these dass li-positive cells. It was also shown that donor cells migrate into the recipient's spieen early after transplantation. The number of these cells was smaller when the transplanted organ was perfused with the mAb. Further studies are suggested to deplete the graft of donor dendritic cells more adequately. They should also combine graft perfusion with antidass II mAb and recipient immunosuppression at reduced doses.
KW  - Chirurgie
KW  - Class II antigen blockade
KW  - rat
KW  - renal transplantation
KW  - Monoclonal antibody
KW  - dass II antigen blockade
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64431
ER  - 
TY  - JOUR
A1  - Kekow, Jörn
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, Wolfgang
A1  - Gross, Wolfgang L.
T1  - Measurement of rat insulin. Enzyme-linked immunosorbent assay with increased sensitivity, high accuracy, and greater practicability than established radioimmunoassay
N2  - Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IR! measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELlSA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle of competitive saturation. In this ELlSA, IRI can be determined in a wide range of concentrations, corresponding to the standards. The standard curve ranges from 100 to 0.049 ng/mllRI (1 ng/ml - 23.4 JLU/ml - 172 pM rat insulin). The statistical analysis shows between- and within-assay coefficients of variation of :515%. Diabetes 37:321-26,1988
KW  - Chirurgie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45543
ER  - 
TY  - JOUR
A1  - Kekow, J.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
A1  - Gross, WL
T1  - Pancreas transplantation: a study of insulin secretion in isolated islets of Langerhans and in sera using a new enzyme-linked immunosorbent assay
N2  - No abstract available
KW  - Chirurgie
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45229
ER  - 
TY  - JOUR
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Influence of porcine strain, age, and pH of the isolation medium on porcine pancreatic islet isolation success
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45171
ER  - 
TY  - JOUR
A1  - Falk, W.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - 15-Deoxyspergualin (a new guanidine-like drug) blocks T lymphocyte proliferation
N2  - No abstract available
KW  - Chirurgie
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45215
ER  - 
TY  - JOUR
A1  - Eckstein, V.
A1  - Ulrichs, K.
A1  - Meincke, G.
A1  - Müller-Ruchholtz, W.
T1  - Natural xenophile antibodies from sera of type I diabetic patients differ strongly in their reactivity against various porcine pancreatic cells
N2  - No abstract available
KW  - Chirurgie
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45277
ER  - 
TY  - JOUR
A1  - Eckstein, V.
A1  - Müller-Buchholtz, W.
A1  - Ulrichs, Karin
T1  - Reaction patterns of natural xenophile antibodies in human sera with pancreatic islet cells and porcine lymphocytes
N2  - No abstract available
KW  - Chirurgie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44742
ER  - 
TY  - JOUR
A1  - Deltz, E.
A1  - Müller-Hermelink, HK
A1  - Ulrichs, Karin
A1  - Thiede, A.
A1  - Müller-Ruchholtz, W.
T1  - Development of graft-versus-host reaction in various target organs after small intestine transplantation
N2  - The GVHRIL foHowing transplantation of small intestine are different from those found after bone marrow transplantation or spleen cell injections in that they show a remarka ble, significant prevalence of lesions within the intestinal mucosa. These findings are consistent with the observation that jntestinal lymphocytes newly formed in mesenteric lymph nodes predominantly home in on the intestine again.& The degree of histologic alteration within different tissues indicates that the graft and the host may survive the lesions of the lymphatic tissues, whereas the severe intestinal lesions following GVHR may easily cause death of the recipient. With regard to clinical sman bowel transplantation two statements can be made: (l) GVHRIL play a significant role in small bowel trans~ plantation. (2) To minimize their biologic importance, a selective elimination of the graft's Jymph nodes by irradiation or surgical resection should be considered in view of the remarkable difference between GVHRIL in lymph nodes and in the graft's intestinal wall itself.
KW  - Chirurgie
Y1  - 1981
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45459
ER  - 
TY  - JOUR
A1  - Breitkreuz, A.
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Müller-Ruchholz, W.
T1  - Long-term suppression of natural and graft-induced xenophile antibodies by short-term antigen-cyclophosphamide treatment
N2  - No abstract available
KW  - Chirurgie
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45262
ER  -