TY  - JOUR
A1  - Van Die, I.
A1  - Kramer, C.
A1  - Hacker, Jörg
A1  - Bergmans, H.
A1  - Jongen, W.
A1  - Hoekstra, W.
T1  - Nucleotide sequence of the genes coding for minor fimbrial subunits of the F1C fimbriae of Escherichia coli
N2  - F 1 C fimbriae allow uropathogenic Escherichia coli to adhere to specific epithelial surfaces. This adhesive property is probably due to the presence of minor fimbrial components in F1C fimbriae. The foe gene cluster encoding F1C fimbriae has been cloned, as described previously. Here we present the nucleotide sequence (2081 bp) coding for the F 1 C minor fimbria I subunits. The structural genes code for polypeptides of 175 (FocF), 166 (FocG), and 300 (FocH) amino acids. The deduced amino acids of the F 1 C minor subunits were compared with the reported sequences of the minor subunits of other types of fimbriae. The data show that the Foc minor subunits are highly homologous to the corresponding Sfa proteins, whereas homology to the minor subunits of type 1 and P fimbriae is much lower.
KW  - Pilus
KW  - Escherichia coli
KW  - Adherence
KW  - Urinary tract
KW  - Foc protein
KW  - Minor subunits
KW  - Sequencing
KW  - Homology
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-40353
ER  - 
TY  - JOUR
A1  - Schmoll, T.
A1  - Morschhäuser, J.
A1  - Ott, M.
A1  - Ludwig, B.
A1  - Van Die, I.
A1  - Hacker, Jörg
T1  - Complete genetic organization and functional aspects of the Escherichia coli S fimbrial adhesin determinant: nucleotide sequence of the genes sfaB, C, D, E, F.
N2  - The S fimbrial adhesin (sfa) determinant of E. co/i comprises nine genes situated on a stretch of 7.9 kilobases (kb) DNA. Here the nucleotide sequence of the genes sfa B and sfaC situated proximal to the main structural gene sfaA is described. Sfa-LacZ fusions show that the two genes are transcribed in opposite directions. The isolation of mutants in the proximal region of the sfa gene cluster, the construction of sfa-phoA gene fusions and subsequent transcomplementation sturlies indicated that the genes sfaB and sfaC play a role in regulation of the sfa determinant. ln addition the nucleotide sequence of the genes sfa D, sfa E and sfa F situated between the genes sfaA and sfaG responsible for S subunit proteins, were determined. lt is suggested that these genes are involved in transport and assembly of fimbrial subunits. Thus the entire genetic organization of the sfa determinant is presented and compared with the gene clusters coding for P fimbriae (pap), F1 C fimbriae (foc) and type I fimbriae ( fim). The evolutionary relationship of fimbrial adhesin determinants is discussed.
KW  - Infektionsbiologie
KW  - Escherichia coli
KW  - S fimbrial adhesin (Sfa)
KW  - genetic organization
KW  - gene regulation
KW  - nucleotide sequence
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59661
ER  -