TY  - JOUR
A1  - Minden, Vanessa
A1  - Schnetger, Bernhard
A1  - Pufal, Gesine
A1  - Leonhardt, Sara D.
T1  - Antibiotic-induced effects on scaling relationships and on plant element contents in herbs and grasses
JF  - Ecology and Evolution
N2  - Plant performance is correlated with element concentrations in plant tissue, which may be impacted by adverse chemical soil conditions. Antibiotics of veterinary origin can adversely affect plant performance. They are released to agricultural fields via grazing animals or manure, taken up by plants and may be stored, transformed or sequestered by plant metabolic processes. We studied the potential effects of three antibiotics (penicillin, sulfadiazine, and tetracycline) on plant element contents (macro- and microelements). Plant species included two herb species (Brassica napus and Capsella bursa-pastoris) and two grass species (Triticum aestivum and Apera spica-venti), representing two crop species and two noncrop species commonly found in field margins, respectively. Antibiotic concentrations were chosen as to reflect in vivo situations, that is, relatively low concentrations similar to those detected in soils. In a greenhouse experiment, plants were raised in soil spiked with antibiotics. After harvest, macro- and microelements in plant leaves, stems, and roots were determined (mg/g). Results indicate that antibiotics can affect element contents in plants. Penicillin exerted the greatest effect both on element contents and on scaling relationships of elements between plant organs. Roots responded strongest to antibiotics compared to stems and leaves. We conclude that antibiotics in the soil, even in low concentrations, lead to low-element homeostasis, altering the scaling relationships between roots and other plant organs, which may affect metabolic processes and ultimately the performance of a plant.
KW  - antibiotics
KW  - homeostasis
KW  - scaling relationships
KW  - standardized major axis regression
KW  - tissue nutrient contents
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-224094
VL  - 8
ER  - 
TY  - JOUR
A1  - Mrestani, Achmed
A1  - Pauli, Martin
A1  - Kollmannsberger, Philip
A1  - Repp, Felix
A1  - Kittel, Robert J.
A1  - Eilers, Jens
A1  - Doose, Sören
A1  - Sauer, Markus
A1  - Sirén, Anna-Leena
A1  - Heckmann, Manfred
A1  - Paul, Mila M.
T1  - Active zone compaction correlates with presynaptic homeostatic potentiation
JF  - Cell Reports
N2  - Neurotransmitter release is stabilized by homeostatic plasticity. Presynaptic homeostatic potentiation (PHP) operates on timescales ranging from minute- to life-long adaptations and likely involves reorganization of presynaptic active zones (AZs). At Drosophila melanogaster neuromuscular junctions, earlier work ascribed AZ enlargement by incorporating more Bruchpilot (Brp) scaffold protein a role in PHP. We use localization microscopy (direct stochastic optical reconstruction microscopy [dSTORM]) and hierarchical density-based spatial clustering of applications with noise (HDBSCAN) to study AZ plasticity during PHP at the synaptic mesoscale. We find compaction of individual AZs in acute philanthotoxin-induced and chronic genetically induced PHP but unchanged copy numbers of AZ proteins. Compaction even occurs at the level of Brp subclusters, which move toward AZ centers, and in Rab3 interacting molecule (RIM)-binding protein (RBP) subclusters. Furthermore, correlative confocal and dSTORM imaging reveals how AZ compaction in PHP translates into apparent increases in AZ area and Brp protein content, as implied earlier.
KW  - active zone
KW  - Bruchpilot
KW  - RIM-binding protein
KW  - compaction
KW  - homeostasis
KW  - presynaptic plasticity
KW  - super-resolution microscopy
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-265497
VL  - 37
IS  - 1
ER  -