TY  - JOUR
A1  - Blum, Carina
A1  - Taskin, Mehmet Berat
A1  - Shan, Junwen
A1  - Schilling, Tatjana
A1  - Schlegelmilch, Katrin
A1  - Teßmar, Jörg
A1  - Groll, Jürgen
T1  - Appreciating the First Line of the Human Innate Immune Defense: A Strategy to Model and Alleviate the Neutrophil Elastase-Mediated Attack toward Bioactivated Biomaterials
JF  - Small
N2  - Biointerface engineering is a wide-spread strategy to improve the healing process and subsequent tissue integration of biomaterials. Especially the integration of specific peptides is one promising strategy to promote the regenerative capacity of implants and 3D scaffolds. In vivo, these tailored interfaces are, however, first confronted with the innate immune response. Neutrophils are cells with pronounced proteolytic potential and the first recruited immune cells at the implant site; nonetheless, they have so far been underappreciated in the design of biomaterial interfaces. Herein, an in vitro approach is introduced to model and analyze the neutrophil interaction with bioactivated materials at the example of nano-bioinspired electrospun surfaces that reveals the vulnerability of a given biointerface design to the contact with neutrophils. A sacrificial, transient hydrogel coating that demonstrates optimal protection for peptide-modified surfaces and thus alleviates the immediate cleavage by neutrophil elastase is further introduced.
KW  - solution electrospinning
KW  - human neutrophil elastase (HNE)
KW  - peptide immobilization
KW  - polymeric matrix
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-257691
VL  - 17
IS  - 13
ER  - 
TY  - JOUR
A1  - Shan, Junwen
A1  - Böck, Thomas
A1  - Keller, Thorsten
A1  - Forster, Leonard
A1  - Blunk, Torsten
A1  - Groll, Jürgen
A1  - Teßmar, Jörg
T1  - TEMPO/TCC as a Chemo Selective Alternative for the Oxidation of Hyaluronic Acid
JF  - Molecules
N2  - Hyaluronic acid (HA)-based hydrogels are very commonly applied as cell carriers for different approaches in regenerative medicine. HA itself is a well-studied biomolecule that originates from the physiological extracellular matrix (ECM) of mammalians and, due to its acidic polysaccharide structure, offers many different possibilities for suitable chemical modifications which are necessary to control, for example, network formation. Most of these chemical modifications are performed using the free acid function of the polymer and, additionally, lead to an undesirable breakdown of the biopolymer’s backbone. An alternative modification of the vicinal diol of the glucuronic acid is oxidation with sodium periodate to generate dialdehydes via a ring opening mechanism that can subsequently be further modified or crosslinked via Schiff base chemistry. Since this oxidation causes a structural destruction of the polysaccharide backbone, it was our intention to study a novel synthesis protocol frequently applied to selectively oxidize the C6 hydroxyl group of saccharides. On the basis of this TEMPO/TCC oxidation, we studied an alternative hydrogel platform based on oxidized HA crosslinked using adipic acid dihydrazide as the crosslinker.
KW  - hyaluronic acid
KW  - oxidation
KW  - hydrogel formation
KW  - Schiff base chemistry
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-248362
SN  - 1420-3049
VL  - 26
IS  - 19
ER  -