TY  - JOUR
A1  - Wanzek, Katharina
A1  - Schwindt, Eike
A1  - Capra, John A.
A1  - Paeschke, Katrin
T1  - Mms1 binds to G-rich regions in Saccharomyces cerevisiae and influences replication and genome stability
JF  - Nucleic Acids Research
N2  - The regulation of replication is essential to preserve genome integrity. Mms1 is part of the E3 ubiquitin ligase complex that is linked to replication fork progression. By identifying Mms1 binding sites genome-wide in Saccharomyces cerevisiae we connected Mms1 function to genome integrity and replication fork progression at particular G-rich motifs. This motif can form G-quadruplex (G4) structures in vitro. G4 are stable DNA structures that are known to impede replication fork progression. In the absence of Mms1, genome stability is at risk at these G-rich/G4 regions as demonstrated by gross chromosomal rearrangement assays. Mms1 binds throughout the cell cycle to these G-rich/G4 regions and supports the binding of Pif1 DNA helicase. Based on these data we propose a mechanistic model in which Mms1 binds to specific G-rich/G4 motif located on the lagging strand template for DNA replication and supports Pif1 function, DNA replication and genome integrity.
KW  - replication
KW  - regulation
KW  - genome integrity
KW  - Saccharomyces cerevisiae
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-170577
VL  - 45
IS  - 13
ER  -