TY  - JOUR
A1  - Chen, Yi-chun
A1  - Gerber, Bertram
T1  - Generalization and discrimination tasks yield concordant measures of perceived distance between odours and their binary mixtures in larval Drosophila
JF  - The Journal of Experimental Biology
N2  - Similarity between odours is notoriously difficult to measure. Widely used behavioural approaches in insect olfaction research are cross-adaptation, masking, as well as associative tasks based on olfactory learning and the subsequent testing for how specific the established memory is. A concern with such memory-based approaches is that the learning process required to establish an odour memory may alter the way the odour is processed, such that measures of perception taken at the test are distorted. The present study was therefore designed to see whether behavioural judgements of perceptual distance are different for two different memory-based tasks, namely generalization and discrimination. We used odour-reward learning in larval Drosophila as a study case. In order to challenge the larvae's olfactory system, we chose to work with binary mixtures and their elements (1-octanol, n-amyl acetate, 3-octanol, benzaldehyde and hexyl acetate). We determined the perceptual distance between each mixture and its elements, first in a generalization task, and then in a discrimination task. It turns out that scores of perceptual distance are correlated between both tasks. A re-analysis of published studies looking at element-to-element perceptual distances in larval reward learning and in adult punishment learning confirms this result. We therefore suggest that across a given set of olfactory stimuli, associative training does not grossly alter the pattern of perceptual distances.
KW  - discrimination
KW  - drosophila melanogaster
KW  - generalization
KW  - memory
KW  - olfaction
KW  - perception
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-121625
VL  - 217
IS  - 12
ER  - 
TY  - JOUR
A1  - Partho, Halder
A1  - Chen, Yi-chun
A1  - Brauckhoff, Janine
A1  - Hofbauer, Alois
A1  - Dabauvalle, Marie-Christine
A1  - Lewandrowski, Urs
A1  - Winkler, Christiane
A1  - Sickmann, Albert
A1  - Buchner, Erich
T1  - Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain
JF  - PLoS One
N2  - The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.
KW  - neuropil
KW  - immunohistochemistry techniques
KW  - gel electrophoresis
KW  - immunoprecipitation
KW  - silver staining
KW  - drosophila melanogaster
KW  - antigen processing and recognition
KW  - hybridomas
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-137957
VL  - 6
IS  - 12
ER  -