TY - JOUR A1 - Heinemann, Anna Sophie A1 - Stalp, Jan Lennart A1 - Bonifacio, João Pedro Pereira A1 - Silva, Filo A1 - Willers, Maike A1 - Heckmann, Julia A1 - Fehlhaber, Beate A1 - Völlger, Lena A1 - Raafat, Dina A1 - Normann, Nicole A1 - Klos, Andreas A1 - Hansen, Gesine A1 - Schmolke, Mirco A1 - Viemann, Dorothee T1 - Silent neonatal influenza A virus infection primes systemic antimicrobial immunity JF - Frontiers in Immunology N2 - Infections with influenza A viruses (IAV) cause seasonal epidemics and global pandemics. The majority of these infections remain asymptomatic, especially among children below five years of age. Importantly, this is a time, when immunological imprinting takes place. Whether early-life infections with IAV affect the development of antimicrobial immunity is unknown. Using a preclinical mouse model, we demonstrate here that silent neonatal influenza infections have a remote beneficial impact on the later control of systemic juvenile-onset and adult-onset infections with an unrelated pathogen, Staphylococcus aureus, due to improved pathogen clearance and clinical resolution. Strategic vaccination with a live attenuated IAV vaccine elicited a similar protection phenotype. Mechanistically, the IAV priming effect primarily targets antimicrobial functions of the developing innate immune system including increased antimicrobial plasma activity and enhanced phagocyte functions and antigen-presenting properties at mucosal sites. Our results suggest a long-term benefit from an exposure to IAV during the neonatal phase, which might be exploited by strategic vaccination against influenza early in life to enforce the host’s resistance to later bacterial infections. KW - neonate KW - influenza A virus KW - influenza vaccination KW - innate immunity training KW - antimicrobial immunity KW - Staphylococcus aureus KW - sepsis Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-304782 VL - 14 ER - TY - JOUR A1 - Humberg, Alexander A1 - Fortmann, Ingmar A1 - Siller, Bastian A1 - Kopp, Matthias Volkmar A1 - Herting, Egbert A1 - Göpel, Wolfgang A1 - Härtel, Christoph T1 - Preterm birth and sustained inflammation: consequences for the neonate JF - Seminars in Immunopathology N2 - Almost half of all preterm births are caused or triggered by an inflammatory process at the feto-maternal interface resulting in preterm labor or rupture of membranes with or without chorioamnionitis (“first inflammatory hit”). Preterm babies have highly vulnerable body surfaces and immature organ systems. They are postnatally confronted with a drastically altered antigen exposure including hospital-specific microbes, artificial devices, drugs, nutritional antigens, and hypoxia or hyperoxia (“second inflammatory hit”). This is of particular importance to extremely preterm infants born before 28 weeks, as they have not experienced important “third-trimester” adaptation processes to tolerate maternal and self-antigens. Instead of a balanced adaptation to extrauterine life, the delicate co-regulation between immune defense mechanisms and immunosuppression (tolerance) to allow microbiome establishment is therefore often disturbed. Hence, preterm infants are predisposed to sepsis but also to several injurious conditions that can contribute to the onset or perpetuation of sustained inflammation (SI). This is a continuing challenge to clinicians involved in the care of preterm infants, as SI is regarded as a crucial mediator for mortality and the development of morbidities in preterm infants. This review will outline the (i) role of inflammation for short-term consequences of preterm birth and (ii) the effect of SI on organ development and long-term outcome. KW - preterm infants KW - sustained inflammation KW - sepsis KW - microbiome KW - neurocognitive outcome KW - chronic pulmonary insufficiency of prematurity Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-235019 SN - 1863-2297 VL - 42 ER - TY - JOUR A1 - Boeckel, Hannah A1 - Karsten, Christian M. A1 - Göpel, Wolfgang A1 - Herting, Egbert A1 - Rupp, Jan A1 - Härtel, Christoph A1 - Hartz, Annika T1 - Increased expression of anaphylatoxin C5a-receptor-1 in neutrophils and natural killer cells of preterm infants JF - International Journal of Molecular Sciences N2 - Preterm infants are susceptible to infection and their defense against pathogens relies largely on innate immunity. The role of the complement system for the immunological vulnerability of preterm infants is less understood. Anaphylatoxin C5a and its receptors C5aR1 and -2 are known to be involved in sepsis pathogenesis, with C5aR1 mainly exerting pro-inflammatory effects. Our explorative study aimed to determine age-dependent changes in the expression of C5aR1 and C5aR2 in neonatal immune cell subsets. Via flow cytometry, we analyzed the expression pattern of C5a receptors on immune cells isolated from peripheral blood of preterm infants (n = 32) compared to those of their mothers (n = 25). Term infants and healthy adults served as controls. Preterm infants had a higher intracellular expression of C5aR1 on neutrophils than control individuals. We also found a higher expression of C5aR1 on NK cells, particularly on the cytotoxic CD56\(^{dim}\) subset and the CD56\(^-\) subset. Immune phenotyping of other leukocyte subpopulations revealed no gestational-age-related differences for the expression of and C5aR2. Elevated expression of C5aR1 on neutrophils and NK cells in preterm infants may contribute to the phenomenon of “immunoparalysis” caused by complement activation or to sustained hyper-inflammatory states. Further functional analyses are needed to elucidate the underlying mechanisms. KW - preterm infants KW - C5a KW - C5aR1 KW - neutrophils KW - NK cells KW - innate immunity KW - sepsis Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-321196 SN - 1422-0067 VL - 24 IS - 12 ER -