TY  - JOUR
A1  - Jordan, Martin C.
A1  - Jäckle, Veronika
A1  - Scheidt, Sebastian
A1  - Gilbert, Fabian
A1  - Hölscher-Doht, Stefanie
A1  - Ergün, Süleyman
A1  - Meffert, Rainer H.
A1  - Heintel, Timo M.
T1  - Trans-obturator cable fixation of open book pelvic injuries
JF  - Scientific Reports
N2  - Operative treatment of ruptured pubic symphysis by plating is often accompanied by complications. Trans-obturator cable fixation might be a more reliable technique; however, have not yet been tested for stabilization of ruptured pubic symphysis. This study compares symphyseal trans-obturator cable fixation versus plating through biomechanical testing and evaluates safety in a cadaver experiment. APC type II injuries were generated in synthetic pelvic models and subsequently separated into three different groups. The anterior pelvic ring was fixed using a four-hole steel plate in Group A, a stainless steel cable in Group B, and a titan band in Group C. Biomechanical testing was conducted by a single-leg-stance model using a material testing machine under physiological load levels. A cadaver study was carried out to analyze the trans-obturator surgical approach. Peak-to-peak displacement, total displacement, plastic deformation and stiffness revealed a tendency for higher stability for trans-obturator cable/band fixation but no statistical difference to plating was detected. The cadaver study revealed a safe zone for cable passage with sufficient distance to the obturator canal. Trans-obturator cable fixation has the potential to become an alternative for symphyseal fixation with less complications.
KW  - anatomy
KW  - medical research
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-261212
VL  - 11
IS  - 1
ER  - 
TY  - JOUR
A1  - Lozovaya, N.
A1  - Gataullina, S.
A1  - Tsintsadze, T.
A1  - Tsintsadze, V.
A1  - Pallesi-Pocachard, E.
A1  - Minlebaev, M.
A1  - Goriounova, N. A.
A1  - Buhler, E.
A1  - Watrin, F.
A1  - Shityakov, S.
A1  - Becker, A. J.
A1  - Bordey, A.
A1  - Milh, M.
A1  - Scavarda, D.
A1  - Bulteau, C.
A1  - Dorfmuller, G.
A1  - Delalande, O.
A1  - Represa, A.
A1  - Cardoso, C.
A1  - Dulac, O.
A1  - Ben-Ari, Y.
A1  - Burnashev, N.
T1  - Selective suppression of excessive GluN2C expression rescues early epilepsy in a tuberous sclerosis murine model
JF  - Nature Communications
N2  - Tuberous sclerosis complex (TSC), caused by dominant mutations in either TSC1 or TSC2 tumour suppressor genes is characterized by the presence of brain malformations, the cortical tubers that are thought to contribute to the generation of pharmacoresistant epilepsy. Here we report that tuberless heterozygote \(Tsc1^{+/-}\) mice show functional upregulation of cortical GluN2C-containing N-methyl-D-aspartate receptors (NMDARs) in an mTOR-dependent manner and exhibit recurrent, unprovoked seizures during early postnatal life (<P19). Seizures are generated intracortically in the granular layer of the neocortex. Slow kinetics of aberrant GluN2C-mediated currents in spiny stellate cells promotes excessive temporal integration of persistent NMDAR-mediated recurrent excitation and seizure generation. Accordingly, specific GluN2C/D antagonists block seizures in \(Tsc1^{+/-}\) mice in vivo and in vitro. Likewise, GluN2C expression is upregulated in TSC human surgical resections, and a GluN2C/D antagonist reduces paroxysmal hyperexcitability. Thus, GluN2C receptor constitutes a promising molecular target to treat epilepsy in TSC patients.
KW  - biological sciences
KW  - medical research
KW  - neuroscience
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-121276
VL  - 5
IS  - 4563
ER  - 
TY  - JOUR
A1  - Luetkens, Karsten Sebastian
A1  - Ergün, Süleyman
A1  - Huflage, Henner
A1  - Kunz, Andreas Steven
A1  - Gietzen, Carsten Herbert
A1  - Conrads, Nora
A1  - Pennig, Lenhard
A1  - Goertz, Lukas
A1  - Bley, Thorsten Alexander
A1  - Gassenmaier, Tobias
A1  - Grunz, Jan-Peter
T1  - Dose reduction potential in cone-beam CT imaging of upper extremity joints with a twin robotic x-ray system
JF  - Scientific Reports
N2  - Cone-beam computed tomography is a powerful tool for 3D imaging of the appendicular skeleton, facilitating detailed visualization of bone microarchitecture. This study evaluated various combinations of acquisition and reconstruction parameters for the cone-beam CT mode of a twin robotic x-ray system in cadaveric wrist and elbow scans, aiming to define the best possible trade-off between image quality and radiation dose. Images were acquired with different combinations of tube voltage and tube current–time product, resulting in five scan protocols with varying volume CT dose indices: full-dose (FD; 17.4 mGy), low-dose (LD; 4.5 mGy), ultra-low-dose (ULD; 1.15 mGy), modulated low-dose (mLD; 0.6 mGy) and modulated ultra-low-dose (mULD; 0.29 mGy). Each set of projection data was reconstructed with three convolution kernels (very sharp [Ur77], sharp [Br69], intermediate [Br62]). Five radiologists subjectively assessed the image quality of cortical bone, cancellous bone and soft tissue using seven-point scales. Irrespective of the reconstruction kernel, overall image quality of every FD, LD and ULD scan was deemed suitable for diagnostic use in contrast to mLD (very sharp/sharp/intermediate: 60/55/70%) and mULD (0/3/5%). Superior depiction of cortical and cancellous bone was achieved in FD\(_{Ur77}\) and LD\(_{Ur77}\) examinations (p < 0.001) with LD\(_{Ur77}\) scans also providing favorable bone visualization compared to FD\(_{Br69}\) and FD\(_{Br62}\) (p < 0.001). Fleiss’ kappa was 0.618 (0.594–0.641; p < 0.001), indicating substantial interrater reliability. In this study, we demonstrate that considerable dose reduction can be realized while maintaining diagnostic image quality in upper extremity joint scans with the cone-beam CT mode of a twin robotic x-ray system. Application of sharper convolution kernels for image reconstruction facilitates superior display of bone microarchitecture.
KW  - medical research
KW  - preclinical research
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-270429
VL  - 11
IS  - 1
ER  - 
TY  - JOUR
A1  - Schierer, Stefan
A1  - Ostalecki, Christian
A1  - Zinser, Elisabeth
A1  - Lamprecht, Ricarda
A1  - Plosnita, Bianca
A1  - Stich, Lena
A1  - Doerrie, Jan
A1  - Lutz, Manfred B
A1  - Schuler, Gerold
A1  - Baur, Andreas S
T1  - Extracellular vesicles from mature dendritic cells (DC) differentiate monocytes into immature DC
JF  - Life Science Alliance
N2  - During inflammation, murine and human monocytes can develop into dendritic cells (DC), but this process is not entirely understood. Here, we demonstrate that extracellular vesicles (EV) secreted by mature human DC (maDC) differentiate peripheral monocytes into immature DC, expressing a unique marker pattern, including 6-sulfo LacNAc (slan), Zbtb46, CD64, and CD14. While EV from both maDC and immature DC differentiated monocytes similar to GM-CSF/IL-4 stimulation, only maDC-EV produced precursors, which upon maturation stimulus developed into T-cell-activating and IL-12p70-secreting maDC. Mechanistically, maDC-EV induced cell signaling through GM-CSF, which was abundant in EV as were IL-4 and other cytokines and chemokines. When injected into the mouse skin, murine maDC-EV attracted immune cells including monocytes that developed activation markers typical for inflammatory cells. Skin-injected EV also reached lymph nodes, causing a similar immune cell infiltration. We conclude that DC-derived EV likely serve to perpetuate an immune reaction and may contribute to chronic inflammation.
KW  - medical research
KW  - immunology
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-228587
VL  - 1
IS  - 6
ER  -