TY  - JOUR
A1  - Salvador, Ellaine
A1  - Köppl, Theresa
A1  - Hörmann, Julia
A1  - Schönhärl, Sebastian
A1  - Bugaeva, Polina
A1  - Kessler, Almuth F.
A1  - Burek, Malgorzata
A1  - Ernestus, Ralf-Ingo
A1  - Löhr, Mario
A1  - Hagemann, Carsten
T1  - Tumor Treating Fields (TTFields) induce cell junction alterations in a human 3D in vitro model of the blood-brain barrier
JF  - Pharmaceutics
N2  - In a recent study, we showed in an in vitro murine cerebellar microvascular endothelial cell (cerebEND) model as well as in vivo in rats that Tumor-Treating Fields (TTFields) reversibly open the blood–brain barrier (BBB). This process is facilitated by delocalizing tight junction proteins such as claudin-5 from the membrane to the cytoplasm. In investigating the possibility that the same effects could be observed in human-derived cells, a 3D co-culture model of the BBB was established consisting of primary microvascular brain endothelial cells (HBMVEC) and immortalized pericytes, both of human origin. The TTFields at a frequency of 100 kHz administered for 72 h increased the permeability of our human-derived BBB model. The integrity of the BBB had already recovered 48 h post-TTFields, which is earlier than that observed in cerebEND. The data presented herein validate the previously observed effects of TTFields in murine models. Moreover, due to the fact that human cell-based in vitro models more closely resemble patient-derived entities, our findings are highly relevant for pre-clinical studies.
KW  - blood-brain barrier
KW  - Tumor-Treating Fields (TTFields)
KW  - CNS disorders
KW  - human brain microvascular endothelial cells (HBMVEC)
KW  - human cells
KW  - 3D in vitro model
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-304830
SN  - 1999-4923
VL  - 15
IS  - 1
ER  -