TY  - JOUR
A1  - Gillitzer, Reinhard
A1  - Berger, Rudolf
A1  - Moll, Heidrun
T1  - A reliable method for simultaneous demonstration of two antigens using a novel combination of immunogold-silver staining with immunoenzymatic labeling
N2  - We have developed a reliable and sensitive immunohistochemical staining technique which allows the simultaneous demonstration of two different antigens expressed in or on the same cell (referred to as mixed labeling), together with the evaluation of the general histopathological appearance of the tissue. The staining procedure combines a three-step (streptavidin-biotin) immunogold-silver staining (IGSS) with a three-step immunoenzymatic labeling. For this purpose, we investigated the compatibility ofIGSS with various substrates of peroxidase or alkaline phosphatase (AP). Highly reliable and discernible mixed labeling was achieved only after iniriallabeling with IGSS followed by AP labeling using the substrates naphthol AS-MX phosphate/Fast Blue or naphthol AS-HI phosphate/New Fuchsin, respectively. To ensure utmost specificity, we applied FlTC-conjugated mouse monoclonal antibodies and rabbit anti-FlTC immunoglobulins visualized by AP-labeled immunoglobulins and the respective substrate in a final step. This novel approach provides an excellent means for demonstration of immunocompetent cells and unequivocal determination of the percentage of specific cell subsets in infiltrated tissue. The advantages of this method, as compared with double immunofluorescence or double immunoenzymatic labeling, were investigated and are discussed. (J Histochem Cytochem 38:307-313, 1990)
KW  - Immunohistochemistry; Immunogold-silver staining; FITC- anti-FITC system; Leucocyte subpopulations; Two-color staining
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-31092
ER  - 
TY  - JOUR
A1  - Dandekar, Thomas
A1  - Argos, Patrick
T1  - A novel heterodimeric cysteine protease is required for interleukin 1ß processing in monocytes
N2  - No abstract available
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-29986
ER  - 
TY  - JOUR
A1  - Hünig, Siegfried
A1  - Wehner, Ingeborg
T1  - A convenient synthesis of 2,2',6,6'-Tetramethyl-4,4'-bipyridine and its oxidation to 2,2',6,6'-Tetracarboxy-4,4'-bipyridine
N2  - No abstract available
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-32005
ER  -