TY  - JOUR
A1  - Gillitzer, Reinhard
A1  - Berger, Rudolf
A1  - Moll, Heidrun
T1  - A reliable method for simultaneous demonstration of two antigens using a novel combination of immunogold-silver staining with immunoenzymatic labeling
N2  - We have developed a reliable and sensitive immunohistochemical staining technique which allows the simultaneous demonstration of two different antigens expressed in or on the same cell (referred to as mixed labeling), together with the evaluation of the general histopathological appearance of the tissue. The staining procedure combines a three-step (streptavidin-biotin) immunogold-silver staining (IGSS) with a three-step immunoenzymatic labeling. For this purpose, we investigated the compatibility ofIGSS with various substrates of peroxidase or alkaline phosphatase (AP). Highly reliable and discernible mixed labeling was achieved only after iniriallabeling with IGSS followed by AP labeling using the substrates naphthol AS-MX phosphate/Fast Blue or naphthol AS-HI phosphate/New Fuchsin, respectively. To ensure utmost specificity, we applied FlTC-conjugated mouse monoclonal antibodies and rabbit anti-FlTC immunoglobulins visualized by AP-labeled immunoglobulins and the respective substrate in a final step. This novel approach provides an excellent means for demonstration of immunocompetent cells and unequivocal determination of the percentage of specific cell subsets in infiltrated tissue. The advantages of this method, as compared with double immunofluorescence or double immunoenzymatic labeling, were investigated and are discussed. (J Histochem Cytochem 38:307-313, 1990)
KW  - Immunohistochemistry; Immunogold-silver staining; FITC- anti-FITC system; Leucocyte subpopulations; Two-color staining
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-31092
ER  - 
TY  - JOUR
A1  - Hünig, Siegfried
A1  - Wehner, Ingeborg
T1  - A convenient synthesis of 2,2',6,6'-Tetramethyl-4,4'-bipyridine and its oxidation to 2,2',6,6'-Tetracarboxy-4,4'-bipyridine
N2  - No abstract available
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-32005
ER  - 
TY  - JOUR
A1  - Thoenen, Hans
A1  - Hughes, Richard A.
A1  - Sendtner, Michael
T1  - Trophic support of motoneurons: physiological, pathophysiological, and therapeutic implications.
N2  - No abstract available
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-31746
ER  - 
TY  - JOUR
A1  - Dandekar, Thomas
T1  - Olbers' Paradox (peer-reviewed scientific correspondence)
N2  - No abstract available
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-31672
ER  - 
TY  - JOUR
A1  - Moll, Heidrun
T1  - Development of helper T cell subsets: a central role for interleukin 12
N2  - No abstract available
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-30975
ER  - 
TY  - JOUR
A1  - Scheer, Ulrich
A1  - Thiry, Marc
A1  - Goessens, Guy
T1  - Structure, function and assembly of the nucleolus
N2  - No abstract available
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-32057
ER  - 
TY  - JOUR
A1  - Gessler, Manfred
A1  - Bruns, Gail A.
T1  - Sequence of the WT1 upstream region including the Wit-1 gene
N2  - No abstract available
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-30193
ER  - 
TY  - JOUR
A1  - Dandekar, Thomas
A1  - Argos, P.
T1  - Drug assay using antibody mimics made by molecular imprinting
N2  - No abstract available
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-30003
ER  - 
TY  - JOUR
A1  - Dandekar, Thomas
A1  - Argos, Patrick
T1  - A novel heterodimeric cysteine protease is required for interleukin 1ß processing in monocytes
N2  - No abstract available
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-29986
ER  - 
TY  - JOUR
A1  - Dandekar, Thomas
A1  - Argos, Patrick
T1  - Chaperonin-mediated protein folding at the surface of groEL through a "molten globule" intermediate
N2  - No abstract available
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-29939
ER  - 
TY  - JOUR
A1  - Dandekar, Thomas
A1  - Argos, P.
T1  - The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted \(\alpha\)-helices: Crystal structure of the protein DNA-complex
N2  - No abstract available
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-29866
ER  - 
TY  - JOUR
A1  - Dandekar, Thomas
A1  - Argos, P.
T1  - Three-dimensional structure of the 67k N-terminal Fragment of E.coli DNA Topoisomerase I
N2  - No abstract available
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-29836
ER  - 
TY  - JOUR
A1  - Dandekar, Thomas
A1  - Argos, Patrick
T1  - Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding
N2  - No abstract available
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-29814
ER  - 
TY  - JOUR
A1  - Dandekar, Thomas
T1  - Yeast U3 localization and correct sequence (snR17a) and promotor activity (snR17b) identified by homology search
N2  - No abstract available
KW  - yeast U3 localization
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-29781
ER  - 
TY  - JOUR
A1  - Dandekar, Thomas
T1  - Hefezellen - ein gutes Modell für höhere Zellen
N2  - No abstract available
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-29726
ER  -