TY  - THES
A1  - Blanco Redondo, Beatriz
T1  - Studies of synapsin phosphorylation and characterization of monoclonal antibodies from the Würzburg Hybridoma Library in Drosophila melanogaster
T1  - Untersuchungen der Phosphorylierung von Synapsin und Charakterisierung monoklonaler Antikörper der Würzburg Hybridoma Library in Drosophila melanogaster
N2  - Synapsins are conserved synapse-associated hosphoproteins involved in the fine regulation of neurotransmitter release. The aim of the present project is to study the phosphorylation of synapsins and the distribution of phospho-synapsin in the brain of Drosophila melanogaster.
Three antibodies served as important tools in this work, a monoclonal antibody (3C11/α-Syn) that recognizes all known synapsin isoforms and two antisera against phosphorylated synapsin peptides (antiserum PSyn(S6) against phospho-serine 6 and antiserum PSyn(S464) against phospho-serine 464). These antisera were recently generated in collaboration with Bertram Gerber and  Eurogentec. ...
N2  - Synapsine sind konservierte, Synapsen-assoziierte Phosphoproteine, die an der Feinregulation der Neurotransmitterfreisetzung beteiligt sind. Das Ziel des Projektes ist, die Phosphorylierung der Synapsine und die Verteilung des Phospho-Synapsins im Gehirn von Drosophila melanogaster zu untersuchen.
Aus diesem Grunde wurden drei bestimmte Antikörper in dieser Arbeit verwendet: Ein monoklonaler Antikörper (3C11/α-Syn), der alle bekannten Isoformen von Synapsin erkennt, und zwei Antiseren gegen phosphorylierte Synapsinpeptide (das Antiserum PSyn(S6) gegen Phosphoserin 6 und das Antiserum PSyn(S464) gegen
Phosphoserin 464). Diese Antiseren wurden unlängst in Zusammenarbeit mit Bertram Gerber und Eurogentec hergestellt.
KW  - Synapsine
KW  - neuroscience
KW  - Taufliege
KW  - Monoklonaler Antikörper
KW  - Neurowissenschaften
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-93766
ER  - 
TY  - JOUR
A1  - Buchner, Erich
A1  - Blanco Redondo, Beatriz
A1  - Bunz, Melanie
A1  - Halder, Partho
A1  - Sadanandappa, Madhumala K.
A1  - Mühlbauer, Barbara
A1  - Erwin, Felix
A1  - Hofbauer, Alois
A1  - Rodrigues, Veronica
A1  - VijayRaghavan, K.
A1  - Ramaswami, Mani
A1  - Rieger, Dirk
A1  - Wegener, Christian
A1  - Förster, Charlotte
T1  - Identification and Structural Characterization of Interneurons of the Drosophila Brain by Monoclonal Antibodies of the Würzburg Hybridoma Library
JF  - PLoS ONE
N2  - Several novel synaptic proteins have been identified by monoclonal antibodies (mAbs) of the Würzburg hybridoma library generated against homogenized Drosophila brains, e.g. cysteine string protein, synapse-associated protein of 47 kDa, and Bruchpilot. However, at present no routine technique exists to identify the antigens of mAbs of our library that label only a small number of cells in the brain. Yet these antibodies can be used to reproducibly label and thereby identify these cells by immunohistochemical staining. Here we describe the staining patterns in the Drosophila brain for ten mAbs of the Würzburg hybridoma library. Besides revealing the neuroanatomical structure and distribution of ten different sets of cells we compare the staining patterns with those of antibodies against known antigens and GFP expression patterns driven by selected Gal4 lines employing regulatory sequences of neuronal genes. We present examples where our antibodies apparently stain the same cells in different Gal4 lines suggesting that the corresponding regulatory sequences can be exploited by the split-Gal4 technique for transgene expression exclusively in these cells. The detection of Gal4 expression in cells labeled by mAbs may also help in the identification of the antigens recognized by the antibodies which then in addition to their value for neuroanatomy will represent important tools for the characterization of the antigens. Implications and future strategies for the identification of the antigens are discussed.
KW  - cell staining
KW  - drosophila melanogaster
KW  - gene expression
KW  - hybridomas
KW  - immune serum
KW  - library screening
KW  - monoclonal antibodies
KW  - neurons
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-97109
ER  -