TY  - JOUR
A1  - Schreiber, Laura M.
A1  - Lohr, David
A1  - Baltes, Steffen
A1  - Vogel, Ulrich
A1  - Elabyad, Ibrahim A.
A1  - Bille, Maya
A1  - Reiter, Theresa
A1  - Kosmala, Aleksander
A1  - Gassenmaier, Tobias
A1  - Stefanescu, Maria R.
A1  - Kollmann, Alena
A1  - Aures, Julia
A1  - Schnitter, Florian
A1  - Pali, Mihaela
A1  - Ueda, Yuichiro
A1  - Williams, Tatiana
A1  - Christa, Martin
A1  - Hofmann, Ulrich
A1  - Bauer, Wolfgang
A1  - Gerull, Brenda
A1  - Zernecke, Alma
A1  - Ergün, Süleyman
A1  - Terekhov, Maxim
T1  - Ultra-high field cardiac MRI in large animals and humans for translational cardiovascular research
JF  - Frontiers in Cardiovascular Medicine
N2  - A key step in translational cardiovascular research is the use of large animal models to better understand normal and abnormal physiology, to test drugs or interventions, or to perform studies which would be considered unethical in human subjects. Ultrahigh field magnetic resonance imaging (UHF-MRI) at 7 T field strength is becoming increasingly available for imaging of the heart and, when compared to clinically established field strengths, promises better image quality and image information content, more precise functional analysis, potentially new image contrasts, and as all in-vivo imaging techniques, a reduction of the number of animals per study because of the possibility to scan every animal repeatedly. We present here a solution to the dual use problem of whole-body UHF-MRI systems, which are typically installed in clinical environments, to both UHF-MRI in large animals and humans. Moreover, we provide evidence that in such a research infrastructure UHF-MRI, and ideally combined with a standard small-bore UHF-MRI system, can contribute to a variety of spatial scales in translational cardiovascular research: from cardiac organoids, Zebra fish and rodent hearts to large animal models such as pigs and humans. We present pilot data from serial CINE, late gadolinium enhancement, and susceptibility weighted UHF-MRI in a myocardial infarction model over eight weeks. In 14 pigs which were delivered from a breeding facility in a national SARS-CoV-2 hotspot, we found no infection in the incoming pigs. Human scanning using CINE and phase contrast flow measurements provided good image quality of the left and right ventricle. Agreement of functional analysis between CINE and phase contrast MRI was excellent. MRI in arrested hearts or excised vascular tissue for MRI-based histologic imaging, structural imaging of myofiber and vascular smooth muscle cell architecture using high-resolution diffusion tensor imaging, and UHF-MRI for monitoring free radicals as a surrogate for MRI of reactive oxygen species in studies of oxidative stress are demonstrated. We conclude that UHF-MRI has the potential to become an important precision imaging modality in translational cardiovascular research.
KW  - ultrahigh-field MRI
KW  - large animal models
KW  - translational research
KW  - research infrastructure
KW  - heart
KW  - organoid
KW  - pig
KW  - cardiovascular MRI
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-317398
SN  - 2297-055X
VL  - 10
ER  - 
TY  - JOUR
A1  - Beck, Christine
A1  - Morbach, Henner
A1  - Beer, Meinrad
A1  - Stenzel, Martin
A1  - Tappe, Dennis
A1  - Gattenlöhner, Stefan
A1  - Hofmann, Ulrich
A1  - Raab, Peter
A1  - Girschick, Hermann J.
T1  - Chronic nonbacterial osteomyelitis in childhood: prospective follow-up during the first year of anti-inflammatory treatment
N2  - Introduction: Chronic nonbacterial osteomyelitis (CNO) is an inflammatory disorder of unknown etiology. In children and adolescents CNO predominantly affects the metaphyses of the long bones, but lesions can occur at any site of the skeleton. Prospectively followed cohorts using a standardized protocol in diagnosis and treatment have rarely been reported. Methods: Thirty-seven children diagnosed with CNO were treated with naproxen continuously for the first 6 months. If assessment at that time revealed progressive disease or no further improvement, sulfasalazine and short-term corticosteroids were added. The aims of our short-term follow-up study were to describe treatment response in detail and to identify potential risk factors for an unfavorable outcome. Results: Naproxen treatment was highly effective in general, inducing a symptom-free status in 43% of our patients after 6 months. However, four nonsteroidal anti-inflammatory drug (NSAID) partial-responders were additionally treated with sulfasalazine and short-term corticosteroids. The total number of clinical detectable lesions was significantly reduced. Mean disease activity estimated by the patient/physician and the physical aspect of health-related quality of life including functional ability (global assessment/childhood health assessment questionnaire and childhood health assessment questionnaire) and pain improved significantly. Forty-one percent of our patients showed radiological relapses, but 67% of them were clinically silent. Conclusions: Most children show a favorable clinical course in the first year of anti-inflammatory treatment with NSAIDs. Relapses and new radiological lesions can occur at any time and at any site in the skeleton but may not be clinically symptomatic. Whole-body magnetic resonance imaging proved to be very sensitive for initial and follow-up diagnostics.
KW  - Mikrobiologie
Y1  - 2010
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-67792
ER  -