TY  - JOUR
A1  - Aldejohann, Alexander Maximilian
A1  - Wiese-Posselt, Miriam
A1  - Gastmeier, Petra
A1  - Kurzai, Oliver
T1  - Expert recommendations for prevention and management of Candida auris transmission
JF  - Mycoses
N2  - Candida auris was first described as a yeast pathogen in 2009. Since then, the species has emerged worldwide. In contrast to most other Candida spp., C. auris frequently exhibits multi-drug resistance and is readily transmitted in hospital settings. While most detections so far are from colonised patients, C. auris does cause superficial and life-threatening invasive infections. During management of the first documented C. auris transmission in a German hospital, experts from the National Reference Centers for Invasive Fungal Infections (NRZMyk) and the National Reference Center for Surveillance of Nosocomial Infections screened available literature and integrated available knowledge on infection prevention and C. auris epidemiology and biology to enable optimal containment. Relevant recommendations developed during this process are summarised in this guidance document, intended to assist in management of C. auris transmission and potential outbreak situations. Rapid and effective measures to contain C. auris spread require a multi-disciplinary approach that includes clinical specialists of the affected unit, nursing staff, hospital hygiene, diagnostic microbiology, cleaning staff, hospital management and experts in diagnostic mycology / fungal infections. Action should be initiated in a step-wise process and relevant interventions differ between management of singular C. auris colonised / infected patients and detection of potential C. auris transmission or nosocomial outbreaks.
KW  - Candida auris
KW  - nosocomial transmission
KW  - infection prevention
KW  - expert recommendation
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-318570
VL  - 65
IS  - 6
SP  - 590
EP  - 598
ER  - 
TY  - JOUR
A1  - Sattler, Janko
A1  - Noster, Janina
A1  - Brunke, Anne
A1  - Plum, Georg
A1  - Wiegel, Pia
A1  - Kurzai, Oliver
A1  - Meis, Jacques F.
A1  - Hamprecht, Axel
T1  - Comparison of two commercially available qPCR kits for the detection of Candida auris
JF  - Journal of Fungi
N2  - Candida auris is an emerging pathogen with resistance to many commonly used antifungal agents. Infections with C. auris require rapid and reliable detection methods to initiate successful medical treatment and contain hospital outbreaks. Conventional identification methods are prone to errors and can lead to misidentifications. PCR-based assays, in turn, can provide reliable results with low turnaround times. However, only limited data are available on the performance of commercially available assays for C. auris detection. In the present study, the two commercially available PCR assays AurisID (OLM, Newcastle Upon Tyne, UK) and Fungiplex Candida Auris RUO Real-Time PCR (Bruker, Bremen, Germany) were challenged with 29 C. auris isolates from all five clades and eight other Candida species as controls. AurisID reliably detected C. auris with a limit of detection (LoD) of 1 genome copies/reaction. However, false positive results were obtained with high DNA amounts of the closely related species C. haemulonii, C. duobushaemulonii and C. pseudohaemulonii. The Fungiplex Candida Auris RUO Real-Time PCR kit detected C. auris with an LoD of 9 copies/reaction. No false positive results were obtained with this assay. In addition, C. auris could also be detected in human blood samples spiked with pure fungal cultures by both kits. In summary, both kits could detect C. auris-DNA at low DNA concentrations but differed slightly in their limits of detection and specificity.
KW  - qPCR
KW  - detection limits
KW  - sensitivity
KW  - strain specificity
KW  - commercial kits
KW  - Candida auris
KW  - Fungiplex Candida Auris
KW  - AurisID
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-228879
SN  - 2309-608X
VL  - 7
IS  - 2
ER  -