TY - CHAP A1 - Das, Hirakjyoti A1 - Zografakis, Alexandros A1 - Oeljeklaus, Silke A1 - Warscheid, Bettina T1 - Analysis of Yeast Peroxisomes via Spatial Proteomics T1 - Analyse von Hefeperoxisomen durch Spatial Proteomik T2 - Peroxisomes N2 - Peroxisomes are ubiquitous organelles with essential functions in numerous cellular processes such as lipid metabolism, detoxification of reactive oxygen species and signaling. Knowledge of the peroxisomal proteome including multi-localized proteins and, most importantly, changes of its composition induced by altering cellular conditions or impaired peroxisome biogenesis and function is of paramount importance for a holistic view on peroxisomes and their diverse functions in a cellular context. In this chapter, we provide a spatial proteomics protocol specifically tailored to the analysis of the peroxisomal proteome of baker's yeast that enables the definition of the peroxisomal proteome under distinct conditions and to monitor dynamic changes of the proteome including the relocation of individual proteins to a different cellular compartment. The protocol comprises subcellular fractionation by differential centrifugation followed by Nycodenz density gradient centrifugation of a crude peroxisomal fraction, quantitative mass spectrometric measurements of subcellular and density gradient fractions and advanced computational data analysis, resulting in the establishment of organellar maps on a global scale. KW - peroxisome purification KW - mass spectrometry KW - label-free quantification KW - protein localization KW - spatial proteomics KW - Saccharomyces cerevisiae KW - differential centrifugation KW - density gradient centrifugation KW - organellar mapping Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-327532 PB - Springer ET - accepted version ER - TY - CHAP A1 - Arewa, Olufunmilayo B. A1 - Fakolade, Ayodeji O. ED - Ludwigs, Markus ED - Muriel Ciceri, José Hernán ED - Velling, Annika T1 - Digitalization, Fragmentation, and Justice in Nigeria T2 - Digitalization as a challenge for justice and administration = La digitalización como reto para la justicia y la administración = Digitalisierung als Herausforderung für Justiz und Verwaltung N2 - No abstract available. KW - digitalization KW - justice KW - Nigeria Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-306231 PB - Würzburg University Press CY - Würzburg ER - TY - CHAP A1 - Rodriguez Samudio, Ruben E. ED - Ludwigs, Markus ED - Muriel Ciceri, José Hernán ED - Velling, Annika T1 - E-government Challenges in Smart Societies: The Japanese Experience T2 - Digitalization as a challenge for justice and administration = La digitalizacion como reto para la justicia y la administracion = Digitalisierung als Herausforderung für Justiz und Verwaltung N2 - No abstract available. KW - e-Government KW - Japan Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-306275 PB - Würzburg University Press CY - Würzburg ER - TY - CHAP A1 - Effron, Robin J. ED - Ludwigs, Markus ED - Muriel Ciceri, José Hernán ED - Velling, Annika T1 - Taking Notice and Service of Process Digital T2 - Digitalization as a challenge for justice and administration = La digitalizacion como reto para la justicia y la administracion = Digitalisierung als Herausforderung für Justiz und Verwaltung N2 - No abstract available. KW - Vereinigte Staaten KW - Digitalisierung Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-306297 PB - Würzburg University Press CY - Würzburg ER - TY - CHAP A1 - Jetter, Tobias A1 - Ackermann, Zeno A1 - Snyder-Körber, MaryAnn ED - Jetter, Tobias T1 - Acknowledgements T2 - Global Cultural Studies? Engaged Scholarship between National and Transnational Frames N2 - No abstract available. KW - cultural studies Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-305790 PB - Würzburg University Press CY - Würzburg ER - TY - CHAP A1 - Ackermann, Zeno A1 - Snyder-Körber, MaryAnn ED - Jetter, Tobias T1 - Series Foreword : JMU Cultural Studies – Strategies for Struggling with the Obvious T2 - Global Cultural Studies? Engaged Scholarship between National and Transnational Frames N2 - No abstract available. KW - Kulturwissenschaften KW - Hall, Stuart KW - Globalisierung KW - Kulturwissenschaft KW - cultural studies KW - Nationale Traditionen KW - national traditions KW - Transnationalismus KW - transnationalism Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-305801 PB - Würzburg University Press CY - Würzburg ER - TY - CHAP A1 - Jetter, Tobias ED - Jetter, Tobias T1 - Introduction : Global Cultural Studies? T2 - Global Cultural Studies? Engaged Scholarship between National and Transnational Frames N2 - No abstract available. KW - Kulturwissenschaften KW - Globalisierung KW - Kulturtheorie KW - Kulturpolitik KW - Sozialphilosophie KW - cultural studies KW - globalization KW - cultural theory KW - cultural politics KW - social philosophy Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-305815 PB - Würzburg University Press CY - Würzburg ER - TY - CHAP A1 - Roy, Abhilasha ED - Jetter, Tobias T1 - Countercultures around the World: A Postcolonial Reading of Paul Gilroy’s The Black Atlantic T2 - Global Cultural Studies? Engaged Scholarship between National and Transnational Frames N2 - No abstract available. KW - Kulturwissenschaften KW - Globalisierung KW - Gilroy, Paul: The Black Atlantic KW - Nationale Minderheiten KW - Rassismus KW - cultural studies KW - globalization KW - Gilroy, Paul: The Black Atlantic KW - minorities KW - racism Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-305829 PB - Würzburg University Press CY - Würzburg ER - TY - CHAP A1 - Koch, Louisa ED - Jetter, Tobias T1 - Cultural Studies as a Question of Stance T2 - Global Cultural Studies? Engaged Scholarship between National and Transnational Frames N2 - No abstract available. KW - Kulturwissenschaften KW - Internationalität KW - Alltagskultur KW - Williams, Raymond KW - Spivak, Gayatri Chakravorty KW - cultural studies KW - internationalism KW - ordinary culture KW - Williams, Raymond KW - Spivak, Gayatri Chakravorty Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-305845 PB - Würzburg University Press CY - Würzburg ER - TY - CHAP A1 - Khapai, YS Sochuiwon Priscilla ED - Jetter, Tobias T1 - Rethinking the Culture of Education with Raymond Williams T2 - Global Cultural Studies? Engaged Scholarship between National and Transnational Frames N2 - No abstract available. KW - Kulturwissenschaften KW - Willams, Raymond KW - Alltagskultur KW - Bildung KW - Indien KW - cultural studies KW - Williams, Raymond KW - ordinary culture KW - education KW - India Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-305854 PB - Würzburg University Press CY - Würzburg ER -