TY  - JOUR
A1  - Laine, Romain F.
A1  - Albecka, Anna
A1  - van de Linde, Sebastian
A1  - Rees, Eric J.
A1  - Crump, Colin M.
A1  - Kaminski, Clemens F.
T1  - Structural analysis of herpes simplex virus by optical super-resolution imaging
JF  - Nature Communications
N2  - Herpes simplex virus type-1 (HSV-1) is one of the most widespread pathogens among humans. Although the structure of HSV-1 has been extensively investigated, the precise organization of tegument and envelope proteins remains elusive. Here we use super-resolution imaging by direct stochastic optical reconstruction microscopy (dSTORM) in combination with a model-based analysis of single-molecule localization data, to determine the position of protein layers within virus particles. We resolve different protein layers within individual HSV-1 particles using multi-colour dSTORM imaging and discriminate envelope-anchored glycoproteins from tegument proteins, both in purified virions and in virions present in infected cells. Precise characterization of HSV-1 structure was achieved by particle averaging of purified viruses and model-based analysis of the radial distribution of the tegument proteins VP16, VP1/2 and pUL37, and envelope protein gD. From this data, we propose a model of the protein organization inside the tegument.
KW  - tegument protein pUL36
KW  - fluorescence microscopy
KW  - monoclonal antibodies
KW  - 3-dimensional structure
KW  - type-1
KW  - nuclear pore complex
KW  - reconstruction microscopy
KW  - localization microscopy
KW  - resolution
KW  - envelopment
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-144623
VL  - 6
IS  - 5980
ER  -