TY  - JOUR
A1  - Roier, Sandro
A1  - Leitner, Deborah R.
A1  - Iwashkiw, Jeremy
A1  - Schild-Prüfert, Kristina
A1  - Feldman, Mario F.
A1  - Krohne, Georg
A1  - Reidl, Joachim
A1  - Schild, Stefan
T1  - Intranasal Immunization with Nontypeable Haemophilus influenzae Outer Membrane Vesicles Induces Cross-Protective Immunity in Mice
JF  - PLoS One
N2  - Haemophilus influenzae is a Gram-negative human-restricted bacterium that can act as a commensal and a pathogen of the respiratory tract. Especially nontypeable H. influenzae (NTHi) is a major threat to public health and is responsible for several infectious diseases in humans, such as pneumonia, sinusitis, and otitis media. Additionally, NTHi strains are highly associated with exacerbations in patients suffering from chronic obstructive pulmonary disease. Currently, there is no licensed vaccine against NTHi commercially available. Thus, this study investigated the utilization of outer membrane vesicles (OMVs) as a potential vaccine candidate against NTHi infections. We analyzed the immunogenic and protective properties of OMVs derived from various NTHi strains by means of nasopharyngeal immunization and colonization studies with BALB/c mice. The results presented herein demonstrate that an intranasal immunization with NTHi OMVs results in a robust and complex humoral and mucosal immune response. Immunoprecipitation revealed the most important immunogenic proteins, such as the heme utilization protein, protective surface antigen D15, heme binding protein A, and the outer membrane proteins P1, P2, P5 and P6. The induced immune response conferred not only protection against colonization with a homologous NTHi strain, which served as an OMV donor for the immunization mixtures, but also against a heterologous NTHi strain, whose OMVs were not part of the immunization mixtures. These findings indicate that OMVs derived from NTHi strains have a high potential to act as a vaccine against NTHi infections.
KW  - conjugate
KW  - obstructive pulmonary disease
KW  - vaccine
KW  - vibrio cholerae
KW  - detoxified lipooligosaccharide
KW  - nasopharyngeal colonization
KW  - functional characterization
KW  - growing escherichia coli
KW  - DNA-binding vesicles
KW  - otitis media
KW  - hemophilus influenzae
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-135201
VL  - 7
IS  - 8
ER  - 
TY  - JOUR
A1  - Zoephel, Judith
A1  - Reiher, Wencke
A1  - Rexer, Karl-Heinz
A1  - Kahnt, Jörg
A1  - Wegener, Christian
T1  - Peptidomics of the Agriculturally Damaging Larval Stage of the Cabbage Root Fly Delia radicum (Diptera: Anthomyiidae)
JF  - PLoS One
N2  - The larvae of the cabbage root fly induce serious damage to cultivated crops of the family Brassicaceae. We here report the biochemical characterisation of neuropeptides from the central nervous system and neurohemal organs, as well as regulatory peptides from enteroendocrine midgut cells of the cabbage maggot. By LC-MALDI-TOF/TOF and chemical labelling with 4-sulfophenyl isothiocyanate, 38 peptides could be identified, representing major insect peptide families: allatostatin A, allatostatin C, FMRFamide-like peptides, kinin, CAPA peptides, pyrokinins, sNPF, myosuppressin, corazonin, SIFamide, sulfakinins, tachykinins, NPLP1-peptides, adipokinetic hormone and CCHamide 1. We also report a new peptide (Yamide) which appears to be homolog to an amidated eclosion hormone-associated peptide in several Drosophila species. Immunocytochemical characterisation of the distribution of several classes of peptide-immunoreactive neurons and enteroendocrine cells shows a very similar but not identical peptide distribution to Drosophila. Since peptides regulate many vital physiological and behavioural processes such as moulting or feeding, our data may initiate the pharmacological testing and development of new specific peptide-based protection methods against the cabbage root fly and its larva.
KW  - adult drosophila
KW  - central-nervous-system
KW  - blowfly calliphora-vomitoria
KW  - drosophila melanogaster
KW  - mass spectometry
KW  - feeding behavior
KW  - fruit fly
KW  - functional characterization
KW  - immunoreactive neurons
KW  - neobellieria bullata
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-131727
VL  - 7
IS  - 7
ER  -