TY  - JOUR
A1  - Sputh, Sebastian
A1  - Panzer, Sabine
A1  - Stigloher, Christian
A1  - Terpitz, Ulrich
T1  - Superaufgelöste Mikroskopie: Pilze unter Beobachtung
JF  - BIOspektrum
N2  - The diffraction limit of light confines fluorescence imaging of subcellular structures in fungi. Different super-resolution methods are available for the analysis of fungi that we briefly discuss. We exploit the filamentous fungus Fusarium fujikuroi expressing a YFP-labeled membrane protein showing the benefit of correlative light- and electron microscopy (CLEM), that combines structured illumination microscopy (SIM) and scanning election microscopy (SEM).
KW  - Pilze
KW  - mikroskopische Untersuchung
KW  - Abbe-Limit
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-270014
SN  - 1868-6249
VL  - 27
IS  - 4
ER  - 
TY  - JOUR
A1  - Brunk, Michael
A1  - Sputh, Sebastian
A1  - Doose, Sören
A1  - van de Linde, Sebastian
A1  - Terpitz, Ulrich
T1  - HyphaTracker: An ImageJ toolbox for time-resolved analysis of spore germination in filamentous fungi
JF  - Scientific Reports
N2  - The dynamics of early fungal development and its interference with physiological signals and environmental factors is yet poorly understood. Especially computational analysis tools for the evaluation of the process of early spore germination and germ tube formation are still lacking. For the time-resolved analysis of conidia germination of the filamentous ascomycete Fusarium fujikuroi we developed a straightforward toolbox implemented in ImageJ. It allows for processing of microscopic acquisitions (movies) of conidial germination starting with drift correction and data reduction prior to germling analysis. From the image time series germling related region of interests (ROIs) are extracted, which are analysed for their area, circularity, and timing. ROIs originating from germlings crossing other hyphae or the image boundaries are omitted during analysis. Each conidium/hypha is identified and related to its origin, thus allowing subsequent categorization. The efficiency of HyphaTracker was proofed and the accuracy was tested on simulated germlings at different signal-to-noise ratios. Bright-field microscopic images of conidial germination of rhodopsin-deficient F. fujikuroi mutants and their respective control strains were analysed with HyphaTracker. Consistent with our observation in earlier studies the CarO deficient mutant germinated earlier and grew faster than other, CarO expressing strains.
KW  - bioinformatics
KW  - cell growth
KW  - fungal biology
KW  - microscopy
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-221691
VL  - 8
ER  -