TY  - JOUR
A1  - Dandekar, Thomas
A1  - Fieselmann, Astrid
A1  - Fischer, Eva
A1  - Popp, Jasmin
A1  - Hensel, Michael
A1  - Noster, Janina
T1  - Salmonella—how a metabolic generalist adopts an intracellular lifestyle during infection
JF  - Frontiers in Cellular and Infection Microbiology
N2  - The human-pathogenic bacterium Salmonella enterica adjusts and adapts to different environments while attempting colonization. In the course of infection nutrient availabilities change drastically. New techniques, “-omics” data and subsequent integration by systems biology improve our understanding of these changes. We review changes in metabolism focusing on amino acid and carbohydrate metabolism. Furthermore, the adaptation process is associated with the activation of genes of the Salmonella pathogenicity islands (SPIs). Anti-infective strategies have to take these insights into account and include metabolic and other strategies. Salmonella infections will remain a challenge for infection biology.
KW  - regulation
KW  - virulence
KW  - "-omics"
KW  - metabolism
KW  - Salmonella-containing vacuole (SCV)
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-120686
SN  - 2235-2988
VL  - 4
IS  - 191
ER  - 
TY  - JOUR
A1  - Bensaad, Karim
A1  - Favaro, Elena
A1  - Lewis, Caroline A.
A1  - Peck, Barrie
A1  - Lord, Simon
A1  - Collins, Jennifer M.
A1  - Pinnick, Katherine E.
A1  - Wigfield, Simon
A1  - Buffa, Francesca M.
A1  - Li, Ji-Liang
A1  - Zhang, Qifeng
A1  - Wakelam, Michael J. O.
A1  - Karpe, Fredrik
A1  - Schulze, Almut
A1  - Harris, Adrian L.
T1  - Fatty Acid Uptake and Lipid Storage Induced by HIF-1 alpha Contribute to Cell Growth and Survival after Hypoxia-Reoxygenation
JF  - Cell Reports
N2  - An in vivo model of antiangiogenic therapy allowed us to identify genes upregulated by bevacizumab treatment, including Fatty Acid Binding Protein 3 (FABP3) and FABP7, both of which are involved in fatty acid uptake. In vitro, both were induced by hypoxia in a hypoxia-inducible factor-1 alpha (HIF-1 alpha)-dependent manner. There was a significant lipid droplet (LD) accumulation in hypoxia that was time and O-2 concentration dependent. Knockdown of endogenous expression of FABP3, FABP7, or Adipophilin (an essential LD structural component) significantly impaired LD formation under hypoxia. We showed that LD accumulation is due to FABP3/7-dependent fatty acid uptake while de novo fatty acid synthesis is repressed in hypoxia. We also showed that ATP production occurs via beta-oxidation or glycogen degradation in a cell-type-dependent manner in hypoxia-reoxygenation. Finally, inhibition of lipid storage reduced protection against reactive oxygen species toxicity, decreased the survival of cells subjected to hypoxia-reoxygenation in vitro, and strongly impaired tumorigenesis in vivo.
KW  - inducible factor-I
KW  - binding protein
KW  - triglyceride accumulation
KW  - cancer cell
KW  - complex-III
KW  - beta-oxidation
KW  - metabolism
KW  - lipogenesis
KW  - proliferation
KW  - resistance
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-115162
SN  - 2211-1247
VL  - 9
IS  - 1
ER  -