TY  - JOUR
A1  - Wolter, Steve
A1  - Endesfelder, Ulrike
A1  - Linde, Sebastian van de
A1  - Heilemann, Mike
A1  - Sauer, Markus
T1  - Measuring localization performance of super-resolution algorithms on very active samples
JF  - Optics Express
N2  - Super-resolution fluorescence imaging based on  inglemolecule localization relies critically on the availability of efficient processing algorithms to distinguish, identify, and localize emissions of single fluorophores. In multiple current applications, such as threedimensional, time-resolved or cluster imaging, high densities of fluorophore emissions are common. Here, we provide an analytic tool to test the performance and quality of localization microscopy algorithms and demonstrate that common algorithms encounter difficulties for samples with high fluorophore density. We demonstrate that, for typical single-molecule localization microscopy methods such as dSTORM and the commonly used rapidSTORM scheme, computational precision limits the acceptable density of concurrently active fluorophores to 0.6 per square micrometer and that the number of successfully localized fluorophores per frame is limited to 0.2 per square micrometer.
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-85936
ER  -