TY - JOUR A1 - Horn, Hannes A1 - Keller, Alexander A1 - Hildebrandt, Ulrich A1 - Kämpfer, Peter A1 - Riederer, Markus A1 - Hentschel, Ute T1 - Draft genome of the \(Arabidopsis\) \(thaliana\) phyllosphere bacterium, \(Williamsia\) sp. ARP1 JF - Standards in Genomic Sciences N2 - The Gram-positive actinomycete \(Williamsia\) sp. ARP1 was originally isolated from the \(Arabidopsis\) \(thaliana\) phyllosphere. Here we describe the general physiological features of this microorganism together with the draft genome sequence and annotation. The 4,745,080 bp long genome contains 4434 protein-coding genes and 70 RNA genes. To our knowledge, this is only the second reported genome from the genus \(Williamsia\) and the first sequenced strain from the phyllosphere. The presented genomic information is interpreted in the context of an adaptation to the phyllosphere habitat. KW - arabidopsis thaliana KW - whole genome sequencing KW - adaption KW - Williamsia sp. ARP1 KW - phyllosphere KW - draft genome KW - next generation sequencing KW - assembly KW - annotation Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-146008 VL - 11 IS - 8 ER - TY - JOUR A1 - Horn, Hannes A1 - Hentschel, Ute A1 - Ramadan Abdelmohsen, Usama T1 - Mining Genomes of Three Marine Sponge-Associated Actinobacterial Isolates for Secondary Metabolism JF - Genome Announcements N2 - Here, we report the draft genome sequences of three actinobacterial isolates, Micromonospora sp. RV43, Rubrobacter sp. RV113, and Nocardiopsis sp. RV163 that had previously been isolated from Mediterranean sponges. The draft genomes were analyzed for the presence of gene clusters indicative of secondary metabolism using antiSMASH 3.0 and NapDos pipelines. Our findings demonstrated the chemical richness of sponge-associated actinomycetes and the efficacy of genome mining in exploring the genomic potential of sponge-derived actinomycetes. Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-124887 VL - 3 IS - 5 ER - TY - JOUR A1 - Harjes, Janno A1 - Ryu, Taewoo A1 - Abdelmohsen, Usama Ramadan A1 - Moitinho-Silva, Lucas A1 - Horn, Hannes A1 - Ravasi, Timothy A1 - Hentschel, Ute T1 - Draft Genome Sequence of the Antitrypanosomally Active Sponge-Associated Bacterium Actinokineospora sp. Strain EG49 N2 - The marine sponge-associated bacterium Actinokineospora sp. strain EG49 produces the antitrypanosomal angucycline-like compound actinosporin A. The draft genome of Actinokineospora sp. EG49 has a size of 7.5 megabases and a GC content of 72.8% and contains 6,629 protein-coding sequences (CDS). antiSMASH predicted 996 genes residing in 36 secondary metabolite gene clusters. KW - Strahlenpilze Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-112776 ER - TY - JOUR A1 - Cheng, Cheng A1 - MacIntyre, Lynsey A1 - Ramadan Abdelmohsen, Usama A1 - Horn, Hannes A1 - Polymenakou, Paraskevi N. A1 - Edrada-Ebel, RuAngelie A1 - Hentschel, Ute T1 - Biodiversity, Anti-Trypanosomal Activity Screening, and Metabolomic Profiling of Actinomycetes Isolated from Mediterranean Sponges JF - PLoS One N2 - Marine sponge–associated actinomycetes are considered as promising sources for the discovery of novel biologically active compounds. In the present study, a total of 64 actinomycetes were isolated from 12 different marine sponge species that had been collected offshore the islands of Milos and Crete, Greece, eastern Mediterranean. The isolates were affiliated to 23 genera representing 8 different suborders based on nearly full length 16S rRNA gene sequencing. Four putatively novel species belonging to genera Geodermatophilus, Microlunatus, Rhodococcus and Actinomycetospora were identified based on a 16S rRNA gene sequence similarity of < 98.5% to currently described strains. Eight actinomycete isolates showed bioactivities against Trypanosma brucei brucei TC221 with half maximal inhibitory concentration (IC50) values <20 μg/mL. Thirty four isolates from the Milos collection and 12 isolates from the Crete collection were subjected to metabolomic analysis using high resolution LC-MS and NMR for dereplication purposes. Two isolates belonging to the genera Streptomyces (SBT348) and Micromonospora (SBT687) were prioritized based on their distinct chemistry profiles as well as their anti-trypanosomal activities. These findings demonstrated the feasibility and efficacy of utilizing metabolomics tools to prioritize chemically unique strains from microorganism collections and further highlight sponges as rich source for novel and bioactive actinomycetes. KW - streptomyces KW - drug metabolism KW - metabolites KW - ribosomal RNA KW - metabolomics KW - actinobacteria KW - sponges KW - secondary metabolites Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-125138 VL - 10 IS - 9 ER - TY - JOUR A1 - Abdelmohsen, Usama Ramadan A1 - Yang, Chen A1 - Horn, Hannes A1 - Hajjar, Dina A1 - Ravasi, Timothy A1 - Hentschel, Ute T1 - Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity N2 - The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery. KW - PKS I KW - Meeresschwämme KW - PKS II KW - NRPS KW - Red sea KW - sponges KW - actinomycetes KW - bioactivity Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-112882 ER -