TY  - THES
A1  - Baumann, Katrin
T1  - Strukturierungsmethoden für Seidenfibroin-Scaffolds
T1  - Structuring methods for silk fibroin scaffolds
N2  - Seidenfibroin findet hauptsächlich als Zellträgermatrix  im Bereich Tissue engineering Anwendung. In Kombination mit verschiedenen Calciumphosphatphasen kann es als Material zur Knochenregeneration verwendet werden. In dieser Arbeit stelle ich mineralisierte Seidenfibroin-Scaffolds mit kontrollierter Makroporosität vor. Im Vergleich zu anderen Studien lag das Ziel auf der simultanen Gelierung und Mineralisation von Seidenfibroin-Scaffolds durch Einlegen von gefrorenen Seidenfibroin Monolithen in angesäuerte Calciumphophosphat Lösung, was zu einer Präzipitation von Monocalciumphosphat in der Seidenfibroinmatrix führt. Im zweiten Teil wurde eine Umsetzung von eingearbeiteten ß-Tricalciumphosphat-Partikeln erreicht. Des weiteren führte ein kontrollierter Cryostrukturierungsprozess von Seidenfibroin-Scaffolds zu parallel ausgerichteten Poren mit Durchmesser zwischen 30 und 50 µm.
N2  - Silk fibroin is commonly used as scaffold material for tissue engineering applications. In combination with mineralization with different calcium phosphate phases, it can also be applied as material for bone regeneration. Here, we present a study which was performed to produce mineralized silk fibroin scaffolds with controlled macroporosity. In contrast to former studies, our Approach focused on a simultaneous gelation and mineralization of silk fibroin by Immersion of frozen silk fibroin Monoliths in acidic calcium Phosphate Solutions. This was achieved by thawing frozen silk fibroin Monoliths in acidic calcium Phosphate solution, leading to the precipitation of monocalcium phosphate within the silk fibroin matrix. In the second approach, a conversion of incorporated ß-tricalcium Phosphate particles into brushite was successfully achieved. Furthermore, a controlled cryostructuring process of silk fibroin scaffolds was carried out of leading to the Formation of parallel oriented pores with Diameters of 30-50 µm.
KW  - Seidenfibroin
KW  - Bruschit
KW  - Mineralisation
KW  - Calciumphosphat
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-159508
ER  - 
TY  - JOUR
A1  - Rödel, Michaela
A1  - Baumann, Katrin
A1  - Groll, Jürgen
A1  - Gbureck, Uwe
T1  - Simultaneous structuring and mineralization of silk fibroin scaffolds
JF  - Journal of Tissue Engineering
N2  - Silk fibroin is commonly used as scaffold material for tissue engineering applications. In combination with a mineralization with different calcium phosphate phases, it can also be applied as material for bone regeneration. Here, we present a study which was performed to produce mineralized silk fibroin scaffolds with controlled macroporosity. In contrast to former studies, our approach focused on a simultaneous gelation and mineralization of silk fibroin by immersion of frozen silk fibroin monoliths in acidic calcium phosphate solutions. This was achieved by thawing frozen silk fibroin monoliths in acidic calcium phosphate solution, leading to the precipitation of monocalcium phosphate within the silk fibroin matrix. In the second approach, a conversion of incorporated -tricalcium phosphate particles into brushite was successfully achieved. Furthermore, a controlled cryostructuring process of silk fibroin scaffolds was carried out leading to the formation of parallel-oriented pores with diameters of 30-50 mu m.
KW  - Brushite
KW  - calcium phosphate
KW  - cryostructuring
KW  - hydrogel
KW  - mineralization
KW  - silk fibroin scaffolds
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-226427
VL  - 9
ER  -