TY  - JOUR
A1  - Jacobs, Graeme
A1  - Bock, Stefanie
A1  - Schuch, Anita
A1  - Moschall, Rebecca
A1  - Schrom, Eva-Maria
A1  - Zahn, Juliane
A1  - Reuter, Christian
A1  - Preiser, Wolfgang
A1  - Rethwilm, Axel
A1  - Engelbrecht, Susan
A1  - Krekau, Thomas
A1  - Bodem, Jochen
T1  - Construction of a high titer Infectious HIV-1 subtype C proviral clone from South Africa
N2  - The Human Immunodeficiency Virus type 1 (HIV-1) subtype C is currently the predominant subtype worldwide. Cell culture studies of Sub-Saharan African subtype C proviral plasmids are hampered by the low replication capacity of the resulting viruses, although viral loads in subtype C infected patients are as high as those from patients with subtype B. Here, we describe the sequencing and construction of a new HIV-1 subtype C proviral clone (pZAC), replicating more than one order of magnitude better than the previous subtype C plasmids. We identify the env-region for being the determinant for the higher viral titers and the pZAC Env to be M-tropic. This higher replication capacity does not lead to a higher cytotoxicity compared to previously described subtype C viruses. In addition, the pZAC Vpu is also shown to be able to down-regulate CD4, but fails to fully counteract CD317.
KW  - HIV
KW  - HIV-1; subtype C; proviral plasmid; viral replication; resistance assays; Vpu; CD317; CD4
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-76340
ER  -