TY  - JOUR
A1  - De Giorgi, Valeria
A1  - Buonaguro, Luigi
A1  - Worschech, Andrea
A1  - Tornesello, Maria Lina
A1  - Izzo, Francesco
A1  - Marincola, Francesco M.
A1  - Wang, Ena
A1  - Buonaguro, Franco M.
T1  - Molecular Signatures Associated with HCV-Induced Hepatocellular Carcinoma and Liver Metastasis
JF  - PLoS ONE
N2  - Hepatocellular carcinomas (HCCs) are a heterogeneous group of tumors that differ in risk factors and genetic alterations. In Italy, particularly Southern Italy, chronic hepatitis C virus (HCV) infection represents the main cause of HCC. Using high-density oligoarrays, we identified consistent differences in gene-expression between HCC and normal liver tissue. Expression patterns in HCC were also readily distinguishable from those associated with liver metastases. To characterize molecular events relevant to hepatocarcinogenesis and identify biomarkers for early HCC detection, gene expression profiling of 71 liver biopsies from HCV-related primary HCC and corresponding HCV-positive non-HCC hepatic tissue, as well as gastrointestinal liver metastases paired with the apparently normal peri-tumoral liver tissue, were compared to 6 liver biopsies from healthy individuals. Characteristic gene signatures were identified when normal tissue was compared with HCV-related primary HCC, corresponding HCV-positive non-HCC as well as gastrointestinal liver metastases. Pathway analysis classified the cellular and biological functions of the genes differentially expressed as related to regulation of gene expression and post-translational modification in HCV-related primary HCC; cellular Growth and Proliferation, and Cell-To-Cell Signaling and Interaction in HCV-related non HCC samples; Cellular Growth and Proliferation and Cell Cycle in metastasis. Also characteristic gene signatures were identified of HCV-HCC progression for early HCC diagnosis. 
Conclusions: A diagnostic molecular signature complementing conventional pathologic assessment was identified.
KW  - identification
KW  - hepatitis C virus
KW  - United States
KW  - gene expression
KW  - class I
KW  - endoplasmic reticulum
KW  - motile phenotype
KW  - bladder cancer
KW  - up-regulation
KW  - target
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-131155
VL  - 8
IS  - 2
ER  -