TY  - JOUR
A1  - Sporbert, Anje
A1  - Cseresnyes, Zoltan
A1  - Heidbreder, Meike
A1  - Domaing, Petra
A1  - Hauser, Stefan
A1  - Kaltschmidt, Barbara
A1  - Kaltschmidt, Christian
A1  - Heilemann, Mike
A1  - Widera, Darius
T1  - Simple Method for Sub-Diffraction Resolution Imaging of Cellular Structures on Standard Confocal Microscopes by Three-Photon Absorption of Quantum Dots
JF  - PLoS ONE
N2  - This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts.
KW  - HIV
KW  - stem-cell
KW  - infection
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-130963
VL  - 8
IS  - 5
ER  - 
TY  - JOUR
A1  - Dasari, Prasad
A1  - Shopova, Iordana A.
A1  - Stroe, Maria
A1  - Wartenberg, Dirk
A1  - Martin-Dahse, Hans
A1  - Beyersdorf, Niklas
A1  - Hortschansky, Peter
A1  - Dietrich, Stefanie
A1  - Cseresnyés, Zoltán
A1  - Figge, Marc Thilo
A1  - Westermann, Martin
A1  - Skerka, Christine
A1  - Brakhage, Axel A.
A1  - Zipfel, Peter F.
T1  - Aspf2 From Aspergillus fumigatus Recruits Human Immune Regulators for Immune Evasion and Cell Damage
JF  - Frontiers in Immunology
N2  - The opportunistic fungal pathogen Aspergillus fumigatus can cause life-threatening infections, particularly in immunocompromised patients. Most pathogenic microbes control host innate immune responses at the earliest time, already before infiltrating host immune cells arrive at the site of infection. Here, we identify Aspf2 as the first A. fumigatus Factor H-binding protein. Aspf2 recruits several human plasma regulators, Factor H, factor-H-like protein 1 (FHL-1), FHR1, and plasminogen. Factor H contacts Aspf2 via two regions located in SCRs6–7 and SCR20. FHL-1 binds via SCRs6–7, and FHR1 via SCRs3–5. Factor H and FHL-1 attached to Aspf2-maintained cofactor activity and assisted in C3b inactivation. A Δaspf2 knockout strain was generated which bound Factor H with 28% and FHL-1 with 42% lower intensity. In agreement with less immune regulator acquisition, when challenged with complement-active normal human serum, Δaspf2 conidia had substantially more C3b (>57%) deposited on their surface. Consequently, Δaspf2 conidia were more efficiently phagocytosed (>20%) and killed (44%) by human neutrophils as wild-type conidia. Furthermore, Aspf2 recruited human plasminogen and, when activated by tissue-type plasminogen activator, newly generated plasmin cleaved the chromogenic substrate S2251 and degraded fibrinogen. Furthermore, plasmin attached to conidia damaged human lung epithelial cells, induced cell retraction, and caused matrix exposure. Thus, Aspf2 is a central immune evasion protein and plasminogen ligand of A. fumigatus. By blocking host innate immune attack and by disrupting human lung epithelial cell layers, Aspf2 assists in early steps of fungal infection and likely allows tissue penetration.
KW  - complement
KW  - blocking opsonization
KW  - phagocytosis
KW  - acquisition of host regulators
KW  - immune evasion
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-197013
SN  - 1664-3224
VL  - 9
IS  - 1635
ER  -