TY  - JOUR
A1  - Kreft, Jürgen
A1  - Funke, D.
A1  - Schlesinger, R.
A1  - Lottspeich, F.
A1  - Goebel, Werner
T1  - Purification and characterization of cytolysins from Listeria monocytogenes serovar 4b and Listeria ivanovii
N2  - Several exoproteins from Listeria monocytogenes serovar 4b (NCTC 10527) and Listeria ivanovii (ATCC) 19119, SLCC 2379), respectively, have been purified to homogeneity by thiol-disulfide exchange chromatography and gel filtration. Both strains produce a haemolytic/cytolytic protein of Mr 58 kDa, which has all the properties of a SH-activated cytolysin, the prototype of which is streptolysin 0 (SLO), and this protein has therefore heen termed Iisteriolysin 0 (LLO). In addition a protein of Mr 24 kDa from culture supernatants of L. ivanovii co-purified withLLO. The N-terminal aminoacid sequences of both proteins from L. ivanovii have been determined. By mutagenesis with transposons of Gram-positive origin (Tn916 and TnI545), which have been introduced via conjugation into L. ivanovii, several phenotypic mutants (altered haemolysis on sheep blood agar or lecithinase-negative) were obtained. Results on the properties of these muntants will he presented.
Y1  - 1989
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-47036
ER  - 
TY  - JOUR
A1  - Goebel, Werner
A1  - Kathariou, S.
A1  - Kuhn, M.
A1  - Sokolovic, Z.
A1  - Kreft, Jürgen
A1  - Köhler, S.
A1  - Funke, D.
A1  - Chakraborty, T.
A1  - Leimeister-Wächter, M.
T1  - Hemolysin from Listeria-biochemistry, genetics and function in pathogenesis
N2  - No abstract available
KW  - Biologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60563
ER  -