TY - JOUR A1 - Donat, Ulrike A1 - Rother, Juliane A1 - Schäfer, Simon A1 - Hess, Michael A1 - Härtl, Barbara A1 - Kober, Christina A1 - Langbein-Laugwitz, Johanna A1 - Stritzker, Jochen A1 - Chen, Nanhai G. A1 - Aguilar, Richard J. A1 - Weibel, Stephanie A1 - Szalay, Alandar A. T1 - Characterization of Metastasis Formation and Virotherapy in the Human C33A Cervical Cancer Model JF - PLoS ONE N2 - More than 90% of cancer mortalities are due to cancer that has metastasized. Therefore, it is crucial to intensify research on metastasis formation and therapy. Here, we describe for the first time the metastasizing ability of the human cervical cancer cell line C33A in athymic nude mice after subcutaneous implantation of tumor cells. In this model, we demonstrated a steady progression of lumbar and renal lymph node metastases during tumor development. Besides predominantly occurring lymphatic metastases, we visualized the formation of hematogenous metastases utilizing red fluorescent protein (RFP) expressing C33A-RFP cells. RFP positive cancer cells were found migrating in blood vessels and forming micrometastases in lungs of tumor-bearing mice. Next, we set out to analyze the influence of oncolytic virotherapy in the C33A-RFP model and demonstrated an efficient virus-mediated reduction of tumor size and metastatic burden. These results suggest the C33A-RFP cervical cancer model as a new platform to analyze cancer metastases as well as to test novel treatment options to combat metastases. KW - metastasis KW - renal cancer KW - oncolytic viruses KW - lymph nodes KW - kidneys KW - lung and intrathoracic tumors KW - secondary lung tumors KW - cancer treatment Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-119674 SN - 1932-6203 VL - 9 IS - 6 ER -