TY  - JOUR
A1  - Hacker, Jörg
A1  - Ott, M.
A1  - Schmidt, G.
A1  - Hull, R.
A1  - Goebel, W.
T1  - Molecular cloning of the F8 fimbrial antigen from Escherichia coli
N2  - The genetic determinant coding for the Pspecific F8 fimbriae was cloned from · the chromosome of the Escherichia coli wild-type strain 2980 (018: K5: H5: FlC, F8). The F8 determinant was further subcloned into the Pstl site of pBR322 and a restriction map was established. In a Southern hybridization experiment identity between the chromosomally encoded F8 determinant of 2980 and its cloned Counterpart was demonstrated. The cloned F8 fimbriäe and those of the wild type strain consist of a protein subunit of nearly 20 kDa. F8 fimbriated strains were agglutinated by an F8 polyclonal antiserum, caused mannose-resistant hemagglutination and attached to human uroepi thellal cells. The cloned F8 determinant was weil expressed in a variety of host strains.
KW  - Infektionsbiologie
KW  - Escherichia coli
KW  - antigen
KW  - F8 fimbriae
KW  - gene cloning
Y1  - 1986
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59391
ER  -