TY  - JOUR
A1  - Lohse, M. J.
A1  - Klotz, K.-N.
A1  - Ukena, D.
A1  - Schwabe, U.
T1  - Characterization of \([^3H]\)Phenobarbital Binding to Rat Brain Membranes
JF  - Neuroscience Letters
N2  - The binding of \([^3H]\)phenobarbital to rat brain membranes was studied in order to determine its characteristics and specificity. The binding reaction was rapid and occurred at sites of low affinity. \((K_d = 700 μM)\) and very high density \((B_{max} = 2.7 nmoll/mg protein)\). It was unaffected by temperature changes from O°C to 95°C and was maximal at pH 5. Detergents in low concentrations markedly decreased the binding, apparently without solubilizing the binding sites. It is concluded that the binding of \([^3H]\) phenobarbital is a rather non-specific interaction with the plasma membrane.
KW  - barbiturates
KW  - radioligand binding
KW  - rat brain membranes
Y1  - 1984
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-127894
VL  - 52
ER  - 
TY  - CHAP
A1  - Lohse, M. J.
A1  - Klotz, K.-N.
A1  - Schwabe, U.
A1  - Christalli, G.
A1  - Vittori, S.
A1  - Grifantini, M.
T1  - Pharmacology and Biochemistry of Adenosine Receptors
N2  - Adenosine modulates a variety of physiological functions via membrane-bound receptors. These receptors couple via G proteins to adenylate cyclase and K+channels. The A1 subtype mediates an inhibition of adenylate cyclase and an opening of K+-channels, and the A2 subtype a Stimulation of adenylate cyclase. Both subtypes have been characterized by radioligand binding. This has facilitated the development of agonists and antagonists with more than 1000-fold A1 selectivity. A1-selective photoaffinity labels have been used for the biochemical characterization of A1 receptors and the study of their coupling to adenylate cyclase. Such selective ligands allow the analysis of the involvement of adenosine receptors in physiological functions. Selective interference with adenosine receptors provides new pharmacological tools and eventually new therapeutic approaches to a number of pathophysiological states.
KW  - Adenosinrezeptor
KW  - Pharmakologie
KW  - Toxikologie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86251
ER  -