TY  - JOUR
A1  - Lohse, M. J.
A1  - Klotz, K.-N.
A1  - Ukena, D.
A1  - Schwabe, U.
T1  - Characterization of \([^3H]\)Phenobarbital Binding to Rat Brain Membranes
JF  - Neuroscience Letters
N2  - The binding of \([^3H]\)phenobarbital to rat brain membranes was studied in order to determine its characteristics and specificity. The binding reaction was rapid and occurred at sites of low affinity. \((K_d = 700 μM)\) and very high density \((B_{max} = 2.7 nmoll/mg protein)\). It was unaffected by temperature changes from O°C to 95°C and was maximal at pH 5. Detergents in low concentrations markedly decreased the binding, apparently without solubilizing the binding sites. It is concluded that the binding of \([^3H]\) phenobarbital is a rather non-specific interaction with the plasma membrane.
KW  - barbiturates
KW  - radioligand binding
KW  - rat brain membranes
Y1  - 1984
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-127894
VL  - 52
ER  -