TY  - JOUR
A1  - Helmprobst, Frederik
A1  - Kneitz, Susanne
A1  - Klotz, Barbara
A1  - Naville, Magali
A1  - Dechaud, Corentin
A1  - Volff, Jean-Nicolas
A1  - Schartl, Manfred
T1  - Differential expression of transposable elements in the medaka melanoma model
JF  - PLoS One
N2  - Malignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma model and RNA-sequencing to investigate the differential expression of TEs in wildtype and transgenic fish carrying melanoma. We constructed a medaka-specific TE sequence library and identified TE sequences that were specifically upregulated in tumors. Validation by qRT- PCR confirmed a specific upregulation of a LINE and an LTR element in malignant melanomas of transgenic fish.
KW  - melanoma
KW  - genomics
KW  - transposable elements
KW  - cancer genomics
KW  - malignant tumors
KW  - gene prediction
KW  - human genomics
KW  - retrotransposons
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-260615
VL  - 16
IS  - 10
ER  -