TY  - JOUR
A1  - Ludwig, Nicole
A1  - Werner, Tamara V.
A1  - Backes, Christina
A1  - Trampert, Patrick
A1  - Gessler, Manfred
A1  - Keller, Andreas
A1  - Lenhof, Hans-Peter
A1  - Graf, Norbert
A1  - Meese, Eckart
T1  - Combining miRNA and mRNA Expression Profiles in
Wilms Tumor Subtypes
JF  - International Journal of Mokecular Sciences
N2  - Wilms tumor (WT) is the most common childhood renal cancer. Recent findings of mutations in microRNA (miRNA) processing proteins suggest a pivotal role of miRNAs in WT genesis. We performed miRNA expression profiling of 36 WTs of different subtypes and four normal kidney tissues using microarrays. Additionally, we determined the gene expression profile of 28 of these tumors to identify potentially correlated target genes and affected pathways. We identified 85 miRNAs and 2107 messenger RNAs (mRNA) differentially expressed in blastemal WT, and 266 miRNAs and 1267 mRNAs differentially expressed in regressive subtype. The hierarchical clustering of the samples, using either the miRNA or mRNA profile, showed the clear separation of WT from normal kidney samples, but the miRNA pattern yielded better separation of WT subtypes. A correlation analysis of the deregulated miRNA and mRNAs identified 13,026 miRNA/mRNA pairs with inversely correlated expression, of which 2844 are potential interactions of miRNA and their predicted mRNA targets. We found significant upregulation of miRNAs-183, -301a/b and -335 for the blastemal subtype, and miRNAs-181b, -223 and -630 for the regressive subtype. We found marked deregulation of miRNAs regulating epithelial to mesenchymal transition, especially in the blastemal subtype, and miRNAs influencing chemosensitivity, especially in regressive subtypes. Further research is needed to assess the influence of preoperative chemotherapy and tumor infiltrating lymphocytes on the miRNA and mRNA patterns in WT
KW  - miRNA
KW  - Wilms tumor
KW  - blastemal
KW  - regressive
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-165430
VL  - 17
IS  - 4
ER  - 
TY  - JOUR
A1  - Schmitt, Jana
A1  - Backes, Christina
A1  - Nourkami-Tutdibi, Nasenien
A1  - Leidinger, Petra
A1  - Deutscher, Stephanie
A1  - Beier, Markus
A1  - Gessler, Manfred
A1  - Graf, Norbert
A1  - Lenhof, Hans-Peter
A1  - Keller, Andreas
A1  - Meese, Eckart
T1  - Treatment-independent miRNA signature in blood of wilms tumor patients
JF  - BMC Genomics
N2  - Background
Blood-born miRNA signatures have recently been reported for various tumor diseases. Here, we compared the miRNA signature in Wilms tumor patients prior and after preoperative chemotherapy according to SIOP protocol 2001.
Results
We did not find a significant difference between miRNA signature of both groups. However both, Wilms tumor patients prior and after chemotherapy showed a miRNA signature different from healthy controls. The signature of Wilms tumor patients prior to chemotherapy showed an accuracy of 97.5% and of patients after chemotherapy an accuracy of 97.0%, each as compared to healthy controls.
Conclusion
Our results provide evidence for a blood-born Wilms tumor miRNA signature largely independent of four weeks preoperative chemotherapy treatment.
KW  - miRNA
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-124034
VL  - 13
IS  - 379
ER  -