TY  - JOUR
A1  - Maghami, Mohammad Ghaem
A1  - Dey, Surjendu
A1  - Lenz, Ann-Kathrin
A1  - Höbartner, Claudia
T1  - Repurpsing Antiviral Drugs for Orthogonal RNA-Catalyzed Labeling
JF  - Angewandte Chemie, International Edition
N2  - In vitro selected ribozymes are promising tools for site-specific labeling of RNA. Previously known nucleic acid catalysts attached fluorescently labeled adenosine or guanosine derivatives through 2’,5’-branched phosphodiester bonds to the RNA of interest. Herein, we report new ribozymes that use orthogonal substrates, derived from the antiviral drug tenofovir, and attach bioorthogonal functional groups, as well as affinity handles and fluorescent reporter units through a hydrolytically more stable phosphonate ester linkage. The tenofovir transferase ribozymes were identified by in vitro selection and are orthogonal to nucleotide transferase ribozymes. As genetically encodable functional RNAs, these ribozymes may be developed for potential cellular applications. The orthogonal ribozymes addressed desired target sites in large RNAs in vitro, as shown by fluorescent labeling of E. coli 16S and 23S RNAs in total cellular RNA.
KW  - Antiviral nucleoside analogues
KW  - in vitro selection
KW  - ribozymes
KW  - site-specific RNA labeling
KW  - tenofovir
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-205552
VL  - 59
ER  -