TY  - JOUR
A1  - Taha, Muhamed-Kheir
A1  - Claus, Heike
A1  - Lappann, Martin
A1  - Veyrier, Frédéric J.
A1  - Otto, Andreas
A1  - Becher, Dörte
A1  - Deghmane, Ala-Eddine
A1  - Frosch, Matthias
A1  - Hellenbrand, Wiebke
A1  - Hong, Eva
A1  - du Châtelet, Isabelle Parent
A1  - Prior, Karola
A1  - Harmsen, Dag
A1  - Vogel, Ulrich
T1  - Evolutionary Events Associated with an Outbreak of Meningococcal Disease in Men Who Have Sex with Men
JF  - PLoS ONE
N2  - Meningococci spread via respiratory droplets, whereas the closely related gonococci are transmitted sexually. Several outbreaks of invasive meningococcal disease have been reported in Europe and the United States among men who have sex with men (MSM). We recently identified an outbreak of serogroup C meningococcal disease among MSM in Germany and France. In this study, genomic and proteomic techniques were used to analyze the outbreak isolates. In addition, genetically identical urethritis isolates were recovered from France and Germany and included in the analysis. Genome sequencing revealed that the isolates from the outbreak among MSM and from urethritis cases belonged to a clade within clonal complex 11. Proteome analysis showed they expressed nitrite reductase, enabling anaerobic growth as previously described for gonococci. Invasive isolates from MSM, but not urethritis isolates, further expressed functional human factor H binding protein associated with enhanced survival in a newly developed transgenic mouse model expressing human factor H, a complement regulatory protein. In conclusion, our data suggest that urethritis and outbreak isolates followed a joint adaptation route including adaption to the urogenital tract.
KW  - nitrites
KW  - genome sequencing
KW  - men who have sex with men
KW  - meningococcal disease
KW  - Neisseria meningitidis
KW  - Neisseria gonorrhoeae
KW  - mammalian genomics
KW  - mouse models
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-179870
VL  - 11
IS  - 5
ER  - 
TY  - JOUR
A1  - Harks, Inga
A1  - Jockel-Schneider, Yvonne
A1  - Schlagenhauf, Ulrich
A1  - May, Theodor W.
A1  - Gravemeier, Martina
A1  - Prior, Karola
A1  - Petersilka, Gregor
A1  - Ehmke, Gregor
T1  - Impact of the Daily Use of a Microcrystal Hydroxyapatite Dentifrice on De Novo Plaque Formation and Clinical/Microbiological Parameters of Periodontal Health. A Randomized Trial
JF  - PLoS ONE
N2  - Aim
This 12-week prospective, randomized, double-blind, two-center trial evaluated the impact of a microcrystalline zinc hydroxyapatite (mHA) dentifrice on plaque formation rate (PFR) in chronic periodontitis patients. We hypothesized that mHA precipitates cause delayed plaque development when compared to a fluoridated control (AmF/SnF\(_{2}\)), and therefore would improve periodontal health.

Material & Methods
At baseline and after 4 and 12 weeks, PFR and other clinical and microbiological parameters were recorded. Seventy periodontitis patients received a mHA or AmF/SnF\(_{2}\) dentifrice as daily oral care without hygiene instructions. Four weeks after baseline, participants received full mouth debridement and continued using the dentifrices for another 8 weeks.

Results
Primary outcome PFR did not change statistically significantly from baseline to weeks 4 and 12, neither in mHA (n = 33; 51.7±17.2% vs. 48.5±16.65% vs. 48.4±19.9%) nor in AmF/SnF2-group (n = 34; 52.3±17.5% vs. 52.5±21.3% vs. 46.1±21.8%). Secondary clinical parameters such as plaque control record, gingival index, bleeding on probing, and pocket probing depth improved, but between-group differences were not statistically significant. Microbiological analyses showed similar slight decreases in colony-forming units in both groups.

Conclusion
In patients with mild-to-moderate periodontitis, periodontal therapy and use of a mHA-or AmF/SnF\(_{2}\) dentifrice without instructions induced comparable improvements in periodontal health but did not significantly reduce the PFR.
KW  - microcrystalline zinc hydroxyapatiteimpact
KW  - plaque formation
KW  - periodontal health
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-166853
VL  - 11
IS  - 7
ER  -