TY  - JOUR
A1  - Groß, Uwe
A1  - Amuzu, Sylvarius K.
A1  - de Ciman, Ring
A1  - Kassimova, Iparkhan
A1  - Groß, Lisa
A1  - Rabsch, Wolfgang
A1  - Rosenberg, Ulrike
A1  - Schulze, Marco
A1  - Stich, August
A1  - Zimmermann, Ortrud
T1  - Bacteremia and Antimicrobial Drug Resistance over Time, Ghana
JF  - Emerging Infectious Diseases
N2  - Bacterial distribution and antimicrobial drug resistance were monitored in patients with bacterial bloodstream infections in rural hospitals in Ghana. In 2001-2002 and in 2009, Salmonella enterica serovar Typhi was the most prevalent pathogen. Although most S. enterica serovar Typhi isolates were chloramphenicol resistant, all isolates tested were susceptible to ciprofloxacin.
KW  - Typhoid-fever
KW  - Children
KW  - Surveillance
KW  - Kenya
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-133805
N1  - All material published in Emerging Infectious Diseases is in the public domain and may be used and reprinted without special permission; proper citation, however, is required.
VL  - 17
IS  - 10
ER  - 
TY  - JOUR
A1  - Friedrich, Torben
A1  - Rahmann, Sven
A1  - Weigel, Wilfried
A1  - Rabsch, Wolfgang
A1  - Fruth, Angelika
A1  - Ron, Eliora
A1  - Gunzer, Florian
A1  - Dandekar, Thomas
A1  - Hacker, Joerg
A1  - Mueller, Tobias
A1  - Dobrindt, Ulrich
T1  - High-throughput microarray technology in diagnostics of enterobacteria based on genome-wide probe selection and regression analysis
N2  - The Enterobacteriaceae comprise a large number of clinically relevant species with several individual subspecies. Overlapping virulence-associated gene pools and the high overall genome plasticity often interferes with correct enterobacterial strain typing and risk assessment. Array technology offers a fast, reproducible and standardisable means for bacterial typing and thus provides many advantages for bacterial diagnostics, risk assessment and surveillance. The development of highly discriminative broad-range microbial diagnostic microarrays remains a challenge, because of marked genome plasticity of many bacterial pathogens. Results: We developed a DNA microarray for strain typing and detection of major antimicrobial resistance genes of clinically relevant enterobacteria. For this purpose, we applied a global genome-wide probe selection strategy on 32 available complete enterobacterial genomes combined with a regression model for pathogen classification. The discriminative power of the probe set was further tested in silico on 15 additional complete enterobacterial genome sequences. DNA microarrays based on the selected probes were used to type 92 clinical enterobacterial isolates. Phenotypic tests confirmed the array-based typing results and corroborate that the selected probes allowed correct typing and prediction of major antibiotic resistances of clinically relevant Enterobacteriaceae, including the subspecies level, e.g. the reliable distinction of different E. coli pathotypes. Conclusions: Our results demonstrate that the global probe selection approach based on longest common factor statistics as well as the design of a DNA microarray with a restricted set of discriminative probes enables robust discrimination of different enterobacterial variants and represents a proof of concept that can be adopted for diagnostics of a wide range of microbial pathogens. Our approach circumvents misclassifications arising from the application of virulence markers, which are highly affected by horizontal gene transfer. Moreover, a broad range of pathogens have been covered by an efficient probe set size enabling the design of high-throughput diagnostics.
KW  - Mikroarray
KW  - Enterobacteriaceae
Y1  - 2010
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-67936
ER  -