TY  - JOUR
A1  - Abdelmohsen, Usama Ramadan
A1  - Yang, Chen
A1  - Horn, Hannes
A1  - Hajjar, Dina
A1  - Ravasi, Timothy
A1  - Hentschel, Ute
T1  - Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity
N2  - The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery.
KW  - PKS I
KW  - Meeresschwämme
KW  - PKS II
KW  - NRPS
KW  - Red sea
KW  - sponges
KW  - actinomycetes
KW  - bioactivity
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-112882
ER  - 
TY  - JOUR
A1  - Harjes, Janno
A1  - Ryu, Taewoo
A1  - Abdelmohsen, Usama Ramadan
A1  - Moitinho-Silva, Lucas
A1  - Horn, Hannes
A1  - Ravasi, Timothy
A1  - Hentschel, Ute
T1  - Draft Genome Sequence of the Antitrypanosomally Active Sponge-Associated Bacterium Actinokineospora sp. Strain EG49
N2  - The marine sponge-associated bacterium Actinokineospora sp. strain EG49 produces the antitrypanosomal angucycline-like compound actinosporin A. The draft genome of Actinokineospora sp. EG49 has a size of 7.5 megabases and a GC content of 72.8% and contains 6,629 protein-coding sequences (CDS). antiSMASH predicted 996 genes residing in 36 secondary metabolite gene clusters.
KW  - Strahlenpilze
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-112776
ER  -