TY  - BOOK
A1  - Bock, Stefanie
A1  - Gauch, Fabian
A1  - Giernat, Yannik
A1  - Hillebrand, Frank
A1  - Kozlova, Darja
A1  - Linck, Lisa
A1  - Moschall, Rebecca
A1  - Sauer, Markus
A1  - Schenk, Christian
A1  - Ulrich, Kristina
A1  - Bodem, Jochen
T1  - HIV-1 : Lehrbuch von Studenten für Studenten
T1  - HIV-1 : a textbook for students written by students
N2  - Dies ist ein Lehrbuch über die HIV-1 Replikation, Pathogenese und Therapie. Es richtet sich an Studenten der Biologie und der Medizin, die etwas mehr über HIV erfahren wollen und stellt neben virologischen Themen auch die zellulären Grundlagen dar. Es umfasst den Viruseintritt, die reverse Transkription, Genom-Integration, Transkriptionsregualtion, die Kotrolle des Spleißens, der Polyadenylierung und des RNA-Exportes. Die Darstellung wird abgerundet mit Kapiteln zum intrazellulärem Transport, zu Nef und zum Virusassembly. In zwei weiteren Kapitel wird die HIV-1 Pathogenese und die Therapie besprochen. Zur Lernkontrolle sind den Kapiteln Fragen und auch Klausurfragen angefügt.
KW  - HIV
KW  - Retroviren
KW  - Lehrbuch
KW  - Viren
KW  - Virologie
KW  - Transkription
KW  - RNS
KW  - Therapie
KW  - Pathogenese
KW  - Epidemiologie
KW  - RNA-Export
KW  - Polyadenylierung
KW  - Reverse Transkription
KW  - Transkription
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78980
SN  - 978-3-923959-90-7
ER  - 
TY  - JOUR
A1  - Moschall, Rebecca
A1  - Denk, Sarah
A1  - Erkelenz, Steffen
A1  - Schenk, Christian
A1  - Schaal, Heiner
A1  - Bodem, Jochen
T1  - A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression
JF  - Retrovirology
N2  - Background:
The foamy viral genome encodes four central purine-rich elements localized in the integrase-coding region of pol. Previously, we have shown that the first two of these RNA elements (A and B) are required for protease dimerization and activation. The D element functions as internal polypurine tract during reverse transcription. Peters et al., described the third element (C) as essential for gag expression suggesting that it might serve as an RNA export element for the unspliced genomic transcript.
Results:
Here, we analysed env splicing and demonstrate that the described C element composed of three GAA repeats known to bind SR proteins regulates env splicing, thus balancing the amount of gag/pol mRNAs. Deletion of the C element effectively promotes a splice site switch from a newly identified env splice acceptor to the intrinsically strong downstream localised env 3′ splice acceptor permitting complete splicing of almost all LTR derived transcripts. We provide evidence that repression of this env splice acceptor is a prerequisite for gag expression. This repression is achieved by the C element, resulting in impaired branch point recognition and SF1/mBBP binding. Separating the branch point from the overlapping purine-rich C element, by insertion of only 20 nucleotides, liberated repression and fully restored splicing to the intrinsically strong env 3′ splice site. This indicated that the cis-acting element might repress splicing by blocking the recognition of essential splice site signals.
Conclusions:
The foamy viral purine-rich C element regulates splicing by suppressing the branch point recognition of the strongest env splice acceptor. It is essential for the formation of unspliced gag and singly spliced pol transcripts.
KW  - splice regulation
KW  - foamy viruses
KW  - branch point
KW  - purine-rich element
KW  - RNA export
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-157614
VL  - 14
IS  - 10
ER  -