TY  - JOUR
A1  - Tylek, Tina
A1  - Blum, Carina
A1  - Hrynevich, Andrei
A1  - Schlegelmilch, Katrin
A1  - Schilling, Tatjana
A1  - Dalton, Paul D
A1  - Groll, Jürgen
T1  - Precisely defined fiber scaffolds with 40 μm porosity induce elongation driven M2-like polarization of human macrophages
JF  - Biofabrication
N2  - Macrophages are key players of the innate immune system that can roughly be divided into the pro-inflammatory M1 type and the anti-inflammatory, pro-healing M2 type. While a transient initial pro-inflammatory state is helpful, a prolonged inflammation deteriorates a proper healing and subsequent regeneration. One promising strategy to drive macrophage polarization by biomaterials is precise control over biomaterial geometry. For regenerative approaches, it is of particular interest to identify geometrical parameters that direct human macrophage polarization. For this purpose, we advanced melt electrowriting (MEW) towards the fabrication of fibrous scaffolds with box-shaped pores and precise inter-fiber spacing from 100 μm down to only 40 μm. These scaffolds facilitate primary human macrophage elongation accompanied by differentiation towards the M2 type, which was most pronounced for the smallest pore size of 40 μm. These new findings can be important in helping to design new biomaterials with an enhanced positive impact on tissue regeneration.
KW  - cell elongation
KW  - human macrophages
KW  - melt electrowriting (MEW)
KW  - macrophage polarization
KW  - 3D scaffolds
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-254012
VL  - 12
IS  - 2
ER  - 
TY  - JOUR
A1  - Blum, Carina
A1  - Taskin, Mehmet Berat
A1  - Shan, Junwen
A1  - Schilling, Tatjana
A1  - Schlegelmilch, Katrin
A1  - Teßmar, Jörg
A1  - Groll, Jürgen
T1  - Appreciating the First Line of the Human Innate Immune Defense: A Strategy to Model and Alleviate the Neutrophil Elastase-Mediated Attack toward Bioactivated Biomaterials
JF  - Small
N2  - Biointerface engineering is a wide-spread strategy to improve the healing process and subsequent tissue integration of biomaterials. Especially the integration of specific peptides is one promising strategy to promote the regenerative capacity of implants and 3D scaffolds. In vivo, these tailored interfaces are, however, first confronted with the innate immune response. Neutrophils are cells with pronounced proteolytic potential and the first recruited immune cells at the implant site; nonetheless, they have so far been underappreciated in the design of biomaterial interfaces. Herein, an in vitro approach is introduced to model and analyze the neutrophil interaction with bioactivated materials at the example of nano-bioinspired electrospun surfaces that reveals the vulnerability of a given biointerface design to the contact with neutrophils. A sacrificial, transient hydrogel coating that demonstrates optimal protection for peptide-modified surfaces and thus alleviates the immediate cleavage by neutrophil elastase is further introduced.
KW  - solution electrospinning
KW  - human neutrophil elastase (HNE)
KW  - peptide immobilization
KW  - polymeric matrix
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-257691
VL  - 17
IS  - 13
ER  -